摘要
水解蚕蛹提取氨基酸,制备氨基酸系列产品是开发我国丰富蚕蛹资源最有效的途径之一。在蚕蛹水解过程中,需要对水解液游离氨基酸含量进行检测,以实现工艺控制。常见的游离氨基酸总量及各种游离酸含量检验的方法包括双指示剂甲醛滴定法、电位滴定法、氨基酸自动分析仪法、高效液相色谱法二极管阵列-荧光检测器联用技术等。
Preparation of amino acid products by hydrolysis silkworm chrysalis is one of the most ettectave ways to develop the rich silkworm chrysalis resources in China. In order to control the technological process, the hydrolysis free amino acid contents were detected in the silkworm chrysalis hydrolysis process. This paper summarized several test methods for the detection of total amino acids and each of the free acid content, the double indicator formaldehyde titration, the potential titration, amino acid autoanalyzer, high performance liquid chromatography coupled with a photo diode array detector and an evaporative light scattering detector （HPLC-DAD-ELSD） were employed in the determination.
出处
《食品工程》
2013年第1期11-14,共4页
Food Engineering
关键词
蚕蛹蛋白
水解
游离氨基酸
检测方法
the pupa protein
hydrolysis
amino acids
determination method
