摘要
用海人酸 (Kainic acid,KA) 10 mg/ kg给 Sprague- Dawley (SD)大鼠颈部皮下注射 ,诱发急性癫痫发作 ,在急性癫痫发作后一周 ,用阈下剂量 (5 mg/ kg)的 KA检测癫痫敏感性。同时分别用原位杂交和免疫组化技术检测并发现癫痫敏感性形成大鼠额叶皮层胆囊收缩素原 (PCCK) m RNA明显增加 ;海马结构中 ,靠近海马齿状回颗粒细胞 (DGCs)基底部锥体样胆囊收缩素 (Cholecystokinin,CCK)免疫反应阳性神经元染色明显增强 ,但海马门尖部 CCK免疫反应阳性神经元明显减少。而癫痫敏感性未形成大鼠未见上述变化 。
Kainic acid (KA) 10mg/kg was subcutaneously injected to Sprague Dawley rats and induced acute convulsions. A week later, subconvulsive dose of KA (5mg/kg) was used to detect the seizure susceptibility. At the same time, by immunocytochemistry and hybridization, PCCK mRNA was found increased significantly in frontal cortex of susceptible rats. The CCK IR was enhanced in pyramidal cells near the DGCs of hippocampus and was decreased obviously in the deep region of hilus. These changes were not observed in nonsusceptible rats, which may be relevant to development of seizure susceptibility.
出处
《解剖科学进展》
CAS
2000年第3期249-251,共3页
Progress of Anatomical Sciences
关键词
海人酸
额叶皮层
海马
癫痫
敏感性
CCK
kainic acid
frontal cortex
hippocampus
seizure susceptibility
CCK
