摘要
目的:探讨外周型苯二氮卓受体对海人酸点燃大鼠癫痫敏感性的影响。方法:大鼠随机分为海人酸组和对照组,海人酸组动物注射惊厥剂量海人酸(8mg.kg-1)建立化学点燃模型。注射7d后再注射阈下剂量海人酸(4mg.kg-1),将癫痫敏感性形成的动物作为癫痫敏感性形成组,未形成的动物作为癫痫敏感性未形成组。对照组分别经皮下注射阈下剂量的海人酸及生理盐水作为单独阈下剂量组和空白对照组。观察120min内动物癫痫发作情况,然后动物断头取脑,采用梯度离心技术制备大脑皮质线粒体,利用放射性配基结合实验测定外周型苯二氮卓受体特异结合量。结果:癫痫敏感性形成组大脑皮质线粒体外周型苯二氮卓受体水平显著高于其他3组(P<0.001)。癫痫敏感性形成组大脑皮质线粒体外周型苯二氮卓受体特异结合量与注射阈下剂量海人酸后动物湿狗样抖动次数、癫痫发作潜伏期、癫痫发作最大强度均具有显著相关性(P<0.001)。结论:大脑皮质线粒体外周型苯二氮卓受体水平与海人酸点燃大鼠癫痫敏感性有关。
Objective： To explore the role of peripheral-type benzodiazepine receptors （PBRs） in seizure susceptibility in kainic acid （KA）-kindled rats. Methods： Rats were divided into KAgroup and control group randomly. Chemically kindled model was set up by injection of kainic acid （KA） （8mg. kg^-1 ） in KAgroup rats. Seven days after initial injection, seizure susceptibility was detected with KA （4mg. kg^-1, x. c. ） in rats. The KA--induced seizure rats were divided into susceptible group and nonsusceptible group according to whether the seizuresusceptibility developed in rats. In addition, there were subconvulsive dose alone group and saline control group. Seizure behaviors were investigated for 120 min. Then, mitochondria from cerebral cortex were purified after decapitation. PBRs specific binding was examined by radioligand assay. Results： In susceptible seizure group, specific PBRs binding was significantly higher in cerebral cortex mitochondria than those in any other groups （P〈0. 001 ） and was significantly related to wet dog shivering count, seizure latency and maximal seizure intensity （P〈 0. 001）. Conclusions： The changes of PBRs in mitochondria in cerebral cortex are associated with the seizure susceptibility in KA-induced rat seizure model.
出处
《脑与神经疾病杂志》
2006年第2期114-116,137,共4页
Journal of Brain and Nervous Diseases
关键词
癫痫
敏感性
外周型苯二氮卓受体
线粒体
大鼠
epilepsy
susceptibility
peripheralbenzodiazepine
receptors
mitochondria
rats
