摘要
目的探讨基质金属蛋白酶－９（ＭＭＰ－９）表达与胃癌侵袭转移及预后的关系。方法采用ＳＰ免疫组织化学方法，检测２０例正常胃黏膜上皮。２０例异型增生、１０８例胃癌组织及４７例相应癌转移淋巴结ＭＭＰ－９的表达情况。结果正常胃黏膜和异型增生ＭＭＰ－９阳性表达率分别为２０．０％（４／２０）和７０％（１４／２０），均呈弱阳性表达；胃癌组织ＭＭＰ－９表达阳性率为８８．９％（９６／１０８），显著高于前两者（Ｐ＜０．０５）；癌转移淋巴结ＭＭＰ－９阳性表达率为１００％（４７／４７），明显高于胃癌原发灶（Ｐ＜０．０５）；ＭＭＰ－９表达强度与胃癌细胞分化程度无明显相关（Ｐ＞０．０５），与胃癌生长方式、浸润深度、淋巴结转移、远处转移、脉管侵犯及病期密切相关（均Ｐ＜０．０５；ＭＭＰ－９强阳性表达胃癌患者术后５年生存率明显下降（Ｐ＜０．０５）。结论ＭＭＰ－ ９表达与胃癌侵袭转移及预后密切相关。
Objective To evaluate the relationship between matrix metalloproteinase - 9(MMP-9) expression and invasion, metastasis and prognosis of gastric cancer. Methods The expression of MMP-9 was detected immunohistochemically in 20 normal gastric mucosa, 20 dysplastic gastric mucosa, 108 gastric cancers tissues and 47 lymph nodes with corresponding cancer metastasis. Results The positive rate of MMP-9 expression was 20.0% (4/20) and 70. 0% (14/20) in normal and dysplastic mucosa respectively, and their positive expressions were weak. The positive tale of MMP-9 immunoreachvity was 88.9% (96/108) and 100.0% (47/47) in primary gastric cancers and metastatic lymph nodes respectively, and the positive rate of MMP-9 in gastric cancer was significantly higher than that in normal and dysplastic mucosa (P<0.05). The staining intensity of MMP-9 was correlated with Ming's tumor growth pattern, the UICC TNM classification, lymphatic and venous vessel invasion and distant metastasis(P< 0.05). The patients with MMP-9 strong positive tumor had low 5-year survival rate(P> 0.05). Conclusion The MMP-9 expression is significantly correlated with invasion, metastasis and prognosis of gastric cancer.
关键词
胃肿瘤
基质金属蛋白酶
预后
Stomach neoplasm Matrix metalloproteinase Prognosis
