摘要
目的探讨中药长必安对人大肠癌细胞表面CD44 表达的影响。方法按长必安含药小鼠血清作用浓度 ,将两株大肠癌细胞各自分别分成低、中、高三组 ,同时设阴性对照组、阳性对照组和长必安与5 -Fu联合用药组。将FITC标记鼠抗人CD44 抗体与上述各组细胞共同孵育后 ,用流式细胞仪检测各组细胞表面CD44 阳性百分率。结果中药长必安作用的各组细胞其CD44 的阳性率较阴性对照组和阳性对照组都有显著下降。结论长必安不仅能抑制大肠癌细胞的体外生长 ,还能有效地降低大肠癌细胞表面的CD44 表达 ,提示在临床应用中 ,长必安在直接抑制肿瘤的同时 ,还能调节机体的免疫应答和免疫状态。
Objective To study the effects of TCM herbs Chang bi an on the expression of CD44 on the surface of human colorectal adenocarcinoma cell lines. Methods Two colorectal carcinoma cell lines were divided into three Chang bi an treated groups: low Chang bi an group, middle group and high group.Negative and positive controls were added to the study. Combination of and 5-FU served as the sixth group. The positive percentage of CD44 was detected with Flow-cytometre after the incubation of colorectal cells and mouse antibodies of anti-human CD44 marked by FITC. Results The positive rate of CD44 in low, middle and high treatad groups and the combination group of and 5-FU were evidently lower than that of positive and negative control groups. Conclusion could not only inhibit the growth of colorectal adenocarcinoma cells in vitro, but also reduce the expression of CD44 on surfaces of coloreactal adenocarcinoma cells. The results suggested that could directly kill the cancer cells and regulate the immunocompetence of the patients.
出处
《浙江临床医学》
2000年第10期657-658,共2页
Zhejiang Clinical Medical Journal
基金
"九五"国家中医药科技攻关课题!基金资助项目 (969060703)
关键词
中药
长必安
CD44
人肠癌细胞系
Changbian Traditional Chinese Medicine CD44 Cell line Human adenocarcinom8
