摘要
目的:观察雷公藤甲素对大肠癌SW480细胞生长增殖、细胞周期及凋亡的影响。方法:体外培养大肠癌SW480细胞,分别给予不同浓度的雷公藤甲素进行干预,采用四甲基耦氮唑蓝(MTT)比色法测定不同浓度药物对SW480细胞生长的抑制率,流式细胞术(FCM)测定SW480细胞周期及凋亡率变化。结果:MTT实验结果显示当药物浓度在20～100 mol/L之间时,体外雷公藤甲素可明显抑制SW480细胞的生长;FCM检测显示药物作用后,细胞凋亡率没有明显变化,但是S期细胞数目显著增多,细胞周期受到明显阻滞。结论:雷公藤甲素对细胞生长有明显的抑制作用,呈现时效-量效关系,其机制可能是使细胞周期发生阻滞。
Objective： To observe the effects of triptolide on the proliferation,cell cycle and apoptosis of colorectal cancer cell line SW480.Methods： SW480 cells were cultured amd treated with triptolide at different concentrations.Inhibition rates of cell growth were detected with MTT assay.The changes of cell cycle and apoptosis rate were detected with flow cytometry（FCM）.Rerults： MTT assay showed that triptolide in the range of 20~100 μmol/L significantly inhibited the proliferation of SW480 cells.FCM showed that no significant difference was found in apoptosis rate before and after treatment,but S phase cells increased significantly and cell cycle was obviously blocked.Conclusions： Triptolide can inhibit SW480 cell proliferation in a dose and time dependent manner,which may be a result of cell cycle arrest.
出处
《贵阳医学院学报》
CAS
2012年第2期141-144,共4页
Journal of Guiyang Medical College
基金
广东高校优秀青年创新人才培育项目(LYM09046)
南方医科大学基础医学院院长基金(JC0905)
关键词
雷公藤
结直肠肿瘤
癌
细胞周期
Tripterigium wilfordii
colorectal neoplasms
carcinoma
cell cycle
