摘要
目的 观察磷脂酶 A2 (phospholipase A2 ,PL A2 )活性变化对高糖或 (和 )高胰岛素 (Ins)环境下培养大鼠血管平滑肌细胞 (vascular smooth muscle cell,VSMC)增殖的影响。方法 在含 2 2m mol· L- 1 Glu或 (和 ) 10 0 Mu· L- 1 Ins的 DMEM培养液中加入 2～ 2 0μmol· L- 1 (终浓度 )的PL A2抑制剂 AACOCF3与 VSMC共同孵育 72 h后 ,用 [3H ]-油酸标记法测定培养上清液中 PL A2活性 ,用细胞计数法和 [3H]-胸腺嘧啶核苷掺入实验观察 VMSC增殖、DNA合成、用流式细胞仪分析 VSMC的细胞周期。结果 AACOCF3浓度为 10～ 2 0 μm ol· L- 1时 ,VSMC的增殖和 DNA合成 ,S期与 G0 +M期细胞百分比 ,抑制剂组均显著低于高糖或 (和 )高 Ins组 (P <0 .0 5 ) ,AACOCF3浓度为 2μm ol· L- 1 时 ,抑制剂组上述指标较高糖或 (和 )高 Ins组下降 ,但差别无显著性 (P >0 .0 5 )。结论 降低 PL A2 活性可抑制高糖或 (和 )高 Ins介导的 VSMC增殖。
Objective This study is to investigate whether phospholipase A 2(PLA 2) activity may affect the proliferation of their vascular smooth muscle cell (VSMC) in cultured rats induced by hyperglycemia and/or hyperinsulinemia. Methods Different concentration of PLA 2 inhibitor(AACOCF 3) was added into VSMC medium which had 22 mmol·L -1 glucose and/or 100 Mu·L -1 insulin, PLA 2 activity was detected by [ 3 H ] Oleate radioactivity(cpm). VSMC was examined by [ 3 H] Tdr incorporation and cell counting. Results AACOCF 3(10～20 μmol·L -1 ) significantly inhibited proliferation of VSMC(P<0.05). AACOCF 3(2 μmol·L -1 ) decreased proliferation of VSMC but no significant(P>0.05). Conclusions Decreasing PLA 2 activity may inhibit VSMC proliferation induced by hyperglycemia and/or hyperinsulinemia.
出处
《疾病控制杂志》
2000年第3期202-204,共3页
Chinese Journal of Disease Control and Prevention
关键词
磷脂酶A2
糖尿病
细胞增殖
动脉粥样硬化
phospholipase A 2
vascular smooth muscles cell
diabetes mellitus
cell proliferation
atherosclerosis
