摘要
为观察葡萄糖和胰岛素对血管平滑肌细胞磷脂酶活性的影响 ,在培养基中分别加入一定浓度的葡萄糖或 (和 )胰岛素与大鼠血管平滑肌细胞共同孵育 72h后 ,分别测定培养上清液中磷脂酶A2 活性和Na+-K+依赖式ATP酶活性 ,磷脂酶A2 活性检测采用 [3 H]—油酸标记法 ,Na+-K+依赖式ATP酶活性检测采用以ATP·2Na为底物的比色法。结果发现大鼠血管平滑肌细胞暴露于高糖或 (和 )高胰岛素环境中 ,上清液中磷脂酶A2 活性增加 ,而Na+-K+依赖式ATP酶活性下降 ,并在一定浓度范围内呈剂量依赖性。葡萄糖浓度由 5 .6mmol L增加到 33.0mmol L ,上清液中磷脂酶A2 活性由 ( 4.2± 0 .2 )× 10 1 2 (min·L)增加到 ( 9.2± 0 .2 )× 10 1 2 (min·L) ;而Na+-K+依赖式ATP酶活性由 4.8± 1.0 2 μmol (g·min)下降到 2 .0 6± 0 .99μmol (g·min)。胰岛素浓度由 5 .0Mu L增加到 10 0 0Mu L ,上清液中磷脂酶A2 活性由 ( 3 .8± 0 .4)× 10 1 2 (min·L)增加到 ( 11.8± 0 .6 )× 10 1 2 (min·L) ;而Na+-K+依赖式ATP酶活性由 4.0± 1.6 8μmol (g·min)下降到 2 .19± 1.0 2 μmol (g·min) ,且进一步发现这一效应在作用 2 4h后才显著 ,高糖和高胰岛素有协同作用。结果提示磷脂酶A2 活性增高与糖尿病时动脉粥样硬化形成有关 ,?
Aim To investigate the effect and significance of glucose and insulin on phospholipase A 2 (PLA 2) activities of vascular smooth muscle cells(VSMC). Methods VSMCs in rats were incubated with the DEME which had different concentration glucose or/and insulin for 72 h PLA 2 activities were detected with [ 3 H]-Oleate radioactivity(cpm). Results PLA 2 activities increased with high concentration of glucose or/and insulin and there were dose-dependent relationship. Conclusion The increased PLA 2 activities were associated with atherosclerosis in diabetes, so treatment with PLA 2 inhibitor could prevent the pathological progress of athcrosclerosis in diabetes.
出处
《中国动脉硬化杂志》
CAS
CSCD
2000年第2期143-146,共4页
Chinese Journal of Arteriosclerosis
