摘要
以文冠果实生苗幼嫩叶片为外植体,研究了植物生长调节剂种类及配比对文冠果组织培养及植株再生的影响,经愈伤诱导、增殖、分化、伸长和生根培养,获得了再生植株,建立了文冠果体细胞胚发生及再生植株体系。结果表明:最适合文冠果胚性愈伤诱导的培养基为DKW+6-BA0.5mg/L+NAA 0.5mg/L+2,4-D 1.0mg/L;最适分化及增殖的培养基为DKW+6-BA0.5mg/L+NAA 0.5mg/L+KT 1.0mg/L;伸长培养基为DKW+6-BA 0.2mg/L+IBA 0.5mg/L;生根培养基为1/2MS+IBA 0.5mg/L,生根率为81%,移栽成活率可达到90%以上。该再生体系的建立为文冠果快速繁殖和遗传转化的研究奠定了基础。
Taking the tender leaves of Xanthoceras sorbifolia as explants,the effect of species and concentrations of plant growth regulator on tissue culture and plant regeneration of Xanthoceras sorbiJblia were studied,after the following four steps：adventitious shoot induction, induction and proliferation and differentiation, root induction, and root growth, an effective regeneration system was established for Xanthoceras sorbifolia. The results showed that the optimal medium for callus induction was DKW＋ 6-BA 0. 5 rag/L＋ NAA 0. 5 rag/L＋ 2,4-D 1.0 mg/L; for callus differentiation and proliferation the optimal medium was DKW-＋-6-BA 0. 5 mg/L＋NAA 0. 5 mg/L＋KT 1.0 nag/L; for elongating DKW＋ 6-BA 0. 2 mg/L＋IBA 0. 5 mg/L, and for rooting, 1/2MS ＋IBA 0. 5 mg/L;the ratio of rooting was 81~,and that of survival was more than 90% when small intact plants were transplanted. The establishment of this regeneration system had laid a foundation for rapid propagation and genetic transformation of Xanthoceras sorbifolia.
出处
《北方园艺》
CAS
北大核心
2013年第7期121-124,共4页
Northern Horticulture
基金
大庆市石油管理局资助项目(dqc-2009-kqfw-ky-001)
关键词
文冠果
愈伤组织
植株再生
Xanthoceras sorbifolia
callus
plant regeneration
