摘要
以猕猴桃(Actindia chinensis)金桃品种的胚乳为外植体,探讨了培养基、生长调节剂和蔗糖浓度以及暗培养等条件对胚乳培养器官形成的影响。结果表明:2,4-D对猕猴桃胚乳愈伤组织的诱导效果显著;在20g/L的蔗糖浓度条件下,胚乳愈伤组织的器官分化效果最好;暗培养可以促进胚乳愈伤组织的生长和器官形成,其中暗培养7d效果最好。
A regeneration protocol for cultured endosperm of Actinidia chinensis were set up via organogenesis from callus tissues derived from endosperm explants of Actinidia chinensis ＇ Jintao＇ in vitro. The effects of different media, plant growth regulators, sucrose concentrations and dark treatment on callus induction and shoot formation were studied. The addition of 2,4-D significantly increased percentage of calli formation form the endosperm explants. Addition of 20g/L of sucrose resulted in better organogenesis form, endosperm callus. Dark culture could promote the growth of endosperm callus and organ formation, and the best efficiency was obtained after 7d of culture in the dark.
出处
《核农学报》
CAS
CSCD
北大核心
2012年第2期257-261,310,共6页
Journal of Nuclear Agricultural Sciences
基金
浙江省自然科学基金项目(Y3080202)
关键词
猕猴桃
愈伤组织
生长调节剂
胚乳培养
Actinidia chinensis
callus tissues
growth regulator
endosperm culture
