摘要
背景:间充质干细胞具有肿瘤靶向的特性,能很容易分离并扩增至需要的数量,间充质干细胞能利用病毒载体进行修饰,提示间充质干细胞在临床肿瘤基因治疗方面的潜力。目的:回顾间充质干细胞的特性、肿瘤趋向性、未修饰的间充质干细胞与肿瘤的关系以及基因修饰的间充质干细胞肿瘤治疗作用。方法:由第一作者通过PubMed数据库检索2002年1月至2012年3月有关间充质干细胞与肿瘤的相关文献。检索词为"Mesenchymalstemcells,tropism,Geneticallymodified,tumor,therapy"。排除重复性研究及无关研究,共保留50篇文章进行综述。结果与结论:间充质干细胞能特异地向多种肿瘤以及它们的转移灶迁移,有研究显示间充质干细胞能促进肿瘤的生长,同时也有研究显示间充质干细胞能抑制肿瘤生长。对间充质干细胞进行修饰进行肿瘤基因治疗的方式主要是携带:自杀基因、凋亡基因、抗血管发生基因、免疫刺激基因或者溶瘤病毒载体,体内外实验证实了这种方法对肿瘤的治疗有效。
BACKGROUND： Mesenchymal stem cells have tumor-targeting properties, and can be isolated easily and expand to the.numbers required for application, and they can be genetically manipulated with viral vectors, which suggest the potential of mesenchymal stem cells in the clinical cancer gene therapy. OBJECTIVE： To review the characteristics of mesenchymal stem cells, the tropism of tumor, the relationships between unmodified mesenchymal stem cells and tumor, and the treatment effect of genetically modified mesenchymal stem cells on tumor. METHODS： A computer-based online search of literatures published between January 2002 to March 2012related to mesenchymal stem cells and tumor was performed in PubMed database by the first author. The key words were ＂mesenchymal stem ceils, tropism, genetically modified, tumor, therapy＂. The repetitive and unrelated researches were excluded, and finally, 50 articles were included according to inclusion criteria. RESULTS AND CONCLUSION： Mesenchymal stem cells can specifically migrate to multiple kinds of tumors and their metastases. Some studies have shown that mesenchymal stem cells can promote the growth of tumor and some other studies have shown that they also inhibit the growth of tumor. The mode to modify the mesenchymal stem cells and perform the tumor gene therapy is mainly to carry the suicide gene, apoptosis genes, anti-angiogenic gene, immune-stimulating genes or oncolytic viral vectors. In vitro and in vivo experiments have confirmed the effect of this method on tumor treatment.
出处
《中国组织工程研究》
CAS
CSCD
2013年第6期1122-1128,共7页
Chinese Journal of Tissue Engineering Research
关键词
干细胞
干细胞综述
间充质干细胞
趋向性
基因修饰
肿瘤
治疗
stem cells
stem cell review
mesenchymal stem cells
tropism
genetically modified
tumor
therapy
