摘要
目的 探讨超顺磁性氧化铁颗粒（SPIO）对于骨髓间充质干细胞（BMSCs）的标记能力以及合适的标记条件。，方法采集成年犬的BMSCs，进行体外培养扩增后，行SPIO标记，观察不同条件下BMSCs的SPIO标记情况及SPIO对BMSCs活性的影响：结果4．5μg／ml及9．0pμg／ml标记组细胞内含有大量的SPIO颗粒。细胞活性未受影响。细胞内铁颗粒的数量随着标记物浓度的增加而增加。18．0pμg／ml标记组细胞内SPIO聚集成团，部分细胞崩解。标记了SPIO的BMSCs达到一定细胞数量（5．0x10。）时，可以存体外磁共振显像（MRI）。随着标记细胞的培养时问延长，细胞内SPIO颗粒逐渐减少（标记1、2、4、8周BMCSs台盼蓝拒染率分别为92．04±1．02、91．08±1．09、90．03±2．03和89．93±1．02），细胞死亡率变化没有统计学意义（p〉0．05）。结论SPIO可以作为一种很好的细胞示踪剂应用于体外细胞示踪。
Objective To explore labeling efficiency and appropriate conditions of Superpara magnetic iron exide nanopaticles （SPIO） nanopartieles for Bone marrow stromal cells（BMSCs）. Methods BMSCs were aquired from skeletally mature dogs via iliae crest aspiration and separated by adherent cell eytopheresis. BMSCs were cultured and incubated with SPIO at different concentrations in vitro. The labeling efficiency of BMSCs with different labeling concentrations SPIO nanoparticles as well as detection of characteristics and signal attenuation rules were evaluated by MRI at 1 .ST in vitro. Results BMSCs were efficiently labeled by SPIO in vitro and has no alterations to viability and proliferation profiles at this labeling concentration. BMSCs loaded with SPIO can be detected by MRI at certainly cell quantity in vitro（5 x 104）. The quantity of SPIO in celis gradually reduced as cell culture time prolonged, with no statistically significant changes in cell death（P 〉 0.05）. Conclusion The results demonstrated the potential application of SPIO as a wonderful cell tracer in vitro.
出处
《中华显微外科杂志》
CSCD
北大核心
2010年第6期457-460,533,共5页
Chinese Journal of Microsurgery
基金
国家自然科学基金资助项目（80772194）
关键词
骨髓间充质干细胞
超顺磁性氧化铁
体外标记
磁共振成像
Bone marrow stromal cells
Superparamagnetic iron oxide
Label in vitro
Magneticresonance imaging
