摘要
目的:研究重组鼠白细胞介素12(rmIL-12)不同给药方案对γ射线急性放射病小鼠的治疗作用。方法:42只BALB/c小鼠均给予60Coγ射线6.0Gy一次全身照射后随机分为对照组、rmIL-12 1次及5次剂量3组,1次剂量组小鼠于照射后1h腹腔注射rmIL-12 20μg/kg,5次剂量组小鼠于照射后1h及此后每3d一次分别腹腔注射rmIL-12 20μg/(kg.d),共5次,对照组给予等体积的无菌PBS。2次/d观察小鼠一般情况,3d检测1次外周血细胞计数,分别于照射后14d和28d收集骨髓细胞进行集落培养。结果:rmIL-12治疗组小鼠一般情况较对照组改善,1及5次剂量组外周血中血小板计数(PLT)下降速度均明显慢于对照组,PLT恢复时间较对照组均明显提前(11d vs 14d),且PLT最低值均明显高于对照组(18.9%vs 8.1%,22.5%vs8.1%,P<0.05),两治疗组间差异无统计学意义(P>0.05);照后17d rmIL-12 1及5次剂量组外周血白细胞恢复速度均快于对照组(P<0.05),两治疗组间差异无统计学意义(P>0.05);照后14d 1及5次剂量组CFU-Mix均明显高于对照组(P<0.01),28d两治疗组CFU-GM、CFU-Mix均高于对照组(P<0.05,P<0.01),两治疗组间差异无统计学意义(P>0.05)。结论:rmIL-12 1及5次剂量组均可明显促进急性放射病小鼠造血系统,尤其是巨核系的恢复,两治疗组效果相当。
Objective：To study the therapeutic effect of different dosing regimens of recombinant routine interleukin 12（ rmIL - 12） on the mice irradiated by γ - rays. Methods： Forty - two BALB/c mice were given 6. OGy 60^Coγ ays total body irradiation and randomly assigned into irradiation control group,rmIL- 12 one dose and five doses treatment group. 20μg/kg of rmIL - 12 was administrated intraper/toneally 1 hour following irradiation in one dose treatment group, and was administrated every 3 days after irradiation for four times additionally in five doses treatment group. The general conditions of mice were observed twice a day, the changes in body weight, peripheral blood cell counts were examined once every three days, bone marrow cells were collected to perform colony cultivation on the 14th and 28th day after irradiation. Results： The general conditions of mice in rmIL - 12 treatment group were better than those of irradiation control group. The decline speed of platelet in rmIL - 12 one and five doses treatment group was significantly slower than that of control group, rmIL - 12 treatment significantly promoted platelet recovery, resulting in less profound nadirs （18.9% vs 8.1% ,22.5% vs 8.1%, P 〈 0. 05）and rapid recovery to normal levels （11 days vs 14 days） ,there was no significant difference between the two treatment groups（ P 〉 O. 05）. The white blood cell recovery speed in two treatment groups was faster than that of the irradiation control group（P 〈 0. 05）, but there was no significant difference between two treatment groups. Semi - solid bone marrow cell culture also demonstrated that rmIL - 12 could stimulate bone marrow cells to form more CFU - Mix than those of the irradiation control group on 14th after irradiation（ P 〈 0.01 ）, and there were more CFU - GM and CFU - Mix in two rmIL - 12 treatment group than those of the irradiation control group on 28th after irradiation （P 〈 0. 05, P 〈 0.01 ）. Conclusion： 1 and 5 dosing regimens of rmIL - 12 can significantly accelerate the recovery of hematopoietic function, especially the recovery of megakaryocyte in acute radiation sickness mice.
出处
《现代肿瘤医学》
CAS
2013年第4期706-709,共4页
Journal of Modern Oncology
基金
全军科技攻关基金资助项目(编号:NO08g157)
关键词
白细胞介素12
急性放射病
造血损伤
γ射线
小鼠
interleukin 12
acute radiation sickness
hematopoietic injury
gamma ray
mice
