摘要
目的研究酪氨酸磷酸酶抑制剂过钒酸钠(Pervanadate,Per)对电离辐射作用后细胞线粒体膜电位(ΔΨm)改变和细胞凋亡的影响。方法应用流式细胞仪测定电离辐射作用后鼠源性髓系白血病细胞株BET-2细胞线粒体膜电位及其在过钒酸钠干预后的改变和细胞凋亡水平。结果电离辐射早期可引起BET-2细胞线粒体膜电位降低,诱导细胞凋亡发生;过钒酸钠在一定程度上稳定细胞线粒体膜电位,减少细胞凋亡。结论过钒酸钠可逆转电离辐射诱导的细胞线粒体膜电位的降低,减少造血细胞凋亡的发生。
Objective To study the effects of pervanadate（Per）, the inhibitor of protein tyrosine phosphotases on the alterations of mitochondrial membrane potential（MMP） and the apoptosis of BET - 2 cells induced by ionizing irradiation. Methods BET - 2 cells were divided into Per - treated groups and non - Per groups and incubated after ionizing irradiation. The MMP and apoptosis were measured with FCM. Results After irradiation was given, the MMP of BET- 2 cells was reduced in the early stage significantly, and the apoptosis appeared, and these changes had a dose - dependent pattern and a time - course fashion. Per potently enhanced the MMP of BET - 2 cells and prevented irradiation - treated cells from apoptosis. Conclusion Per reversed the repression of mitochondrial membrane potential and suppress apoptosis induced by ionizing irradiation in the hematopoietic cells.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2007年第1期62-63,共2页
Chinese Journal of Public Health
基金
江苏省人事厅博士后基金项目(2004-100)
江苏省六大人才高峰资助项目(2005-06)
