摘要
目的 观察高血压左室肥厚消退后心肌细胞跨膜电位的变化。方法 12周龄自发性高血压大鼠 (SHR) ,分别饲服尼群地平或卡托普利 14周 ,以同龄WKY及不经治疗的SHR鼠为对照。采用传统浮置式玻璃微电极记录在体心脏心室肌细胞的动作电位幅度 (APA) ,静息电位 (RMP) ,动作电位时限 (APD) ,及复极 90 %、75 %、5 0 %、2 5 %时的动作电位时限 (APD90 、APD75 、APD5 0 、APD2 5 ) ,并计算APD的离散度。结果 (1)左室肥厚心肌细胞APD及其离散性均明显大于对照组 ;(2 )尼群地平和卡托普利治疗消退左室肥厚后 ,左心室肌细胞APD缩短及APD离散性减少。结论 伴随心肌肥厚的消退 ,心室肌细胞电生理异常好转。
To investigate what the electrophysiological abnormalities in the hypertrophic myocytes were and whether the change in myocyte transmembrane potentials (TP) can be inverted with the regression of LVH Methods Spontaneously Hypertensive Rats (SHRs) and Wistar-Kyoto (WKY) rats of 12 weeks old were applied for this study The rats were divided into six groups ( n =8): WKY-12 group, one SHR-12 group and other four groups, ie (1) SHRs treated with Nitrendipine (30 mg/kg/d, SHR-ni), (2) SHRs treated with Captopril (50 mg/kg/d, SHR-cap), after 14 weeks treatment, the drugs were stopped for one week in these two groups (3) SHRs without treatment (SHR-27), (4) WKY rats of 27 weeks old (WKY-27) Myocyte transmembrane potential in left ventricular epicardial was recorded, and action potential amplitude (APA), resting membrane potential (RMP), action potential duration (APD), and the duration of 90% (APD 90 ), 75% (APD 75 ), 50% (APD 50 ), 25% (APD 25 ) were measured Dispersion of APD was defined as the maximum difference of APDs Results (1) APD, especially APD90 and APD75, was significantly prolonged in LVH myocytes of SHR-12 and SHR-27 than that in WKY rats, and hypertrophied LV showed greater APD dispersion, while RMP and APA did not change significantly (2) After LVH regression made by nitrendipine and captopril, the APD and APD dispersion were shortened No difference of above parameters was found between the two treated groups Conclusion Prolonged APD and obvious APD dispersion in hypertrophic myocytes can be reverted after the regression of LVH
出处
《中国介入心脏病学杂志》
2000年第3期160-162,共3页
Chinese Journal of Interventional Cardiology
关键词
高血压
左室肥厚
消退
跨膜电位
Hypertension
Left ventricular hypertrophy (LVH)
Regression
Transmembrane potential
