摘要
应用ＰＣＲ技术对喉原发癌、转移癌、癌旁正常粘膜和声带息肉石蜡包埋组织中的ＨＰＶＤＮＡ进了检测，结果：４０例喉癌标本中２４例检出ＨＰＶＤＮＡ，阳性率６０％（２４／４０），其中ＨＰＶ６／１１、１６／１８ＤＮＡ阳性者分别为６例（５％）和１８例（４５％）；１２例喉癌颈转移淋巴结６例检出ＨＰＶＤＮＡ，这６例均为ＨＰＶ１６／１８；１０例癌旁正常粘膜和１０例声带息肉均阴性。说明喉癌的发生及其淋巴结转移可能与ＨＰＶ１６／１８感染有关。
To determine the relationship between human papillomavirus (HPV) infection and the development of laryngeal carcinoma,polymerase chain reaction (PCR) technique was used to detect HPV DNA in paraffinembeded specimens of primary laryngeal carcinoma and surrounding normal tissues,cervical metastatic lymphonodi of laryngeal carcinoma,and laryngeal polyps. HPV DNA was found in 24 of 40 (6.0%) primary laryngeal carcinoma ,among which HPV 6/11 and HPV 16/18 were 6 (15 %) and 18 (45%)respectively. HPV 16/18 DNA were also detected in 6 of 12 (50%) metastatic lymphonodi. But no HPV DNA was found in laryngeal polyps and normal tissues. These results suggest that HPV infection may be an etiological factor in the occurrence and development of laryngeal carcinoma.
出处
《河南肿瘤学杂志》
1996年第1期1-3,共3页
Henan Journal of Oncology
关键词
喉癌
人乳头状瘤病毒
聚合酶链反应
laryngeal carcinoma
human papillomavirus (HPV)
polymerase chain reaction (PCR)
