摘要
目的探讨通过siRNA沉默细胞TAp73蛋白表达对骨肉瘤Saos-2细胞的增殖、周期以及侵袭力的作用研究。方法以骨肉瘤Saos-2细胞系为研究对象,设计合成沉默TAp73的siRNA片段,转染入Saos-2细胞,用Western-blot法检测干扰后TAp73蛋白的表达水平,CCK-8法检测细胞增殖,利用流式细胞仪检测细胞周期,Transwell法检测细胞侵袭能力。结果 Western-blot检测显示siRNA对TAp73蛋白表达有显著下调作用;CCK-8检测实验组细胞增殖明显(P<0.05);细胞周期检测显示细胞处于DNA合成期(S期)的细胞比例上升;穿过人工基底膜的细胞数量也明显增加,由(84.17±1.35)增加为(92.67±2.80)(P<0.05)。结论抗癌基因TAp73在骨肉瘤的增殖、凋亡和侵袭过程中发挥有重要作用,可能成为控制肿瘤增殖和转移的关键性因素。
Objective To investigate the pro-apoptosis, anti-migration and anti-proliferation of Saos-2 cells in osteosarcoma through genetic inhibition of expression of TAp73 protein. Methods Human osteosarcoma cell line Saos-2 was cultured. A short hairpin RNA targeting TAp73 was constructed and transfected into Saos-2 cell line. The expression of TAp73 protein were examined by Western blot method. The proliferation was assayed by CCK-8. Flow cytometry was used to detect the cell cycle. Transwell test was used to detect the invasion ability of Saos-2. Results Western blot revealed notably down-regulated expression at protein levels of Tap 73. The cellular proliferation in the treatment group detected by CCK-8 was active after siRNAtreatment （P〈0.05）. The proportion of the cells in the S phase increased. The invasion ability increased from（ 84.17±1.35 ） to （92.67±2.80）（P〈0.01）. Conclusions TAp73 is necessary for human osteosarcoma cell proliferation and invasion. It is probably a potential target for osteosareoma treatment.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2013年第2期170-173,179,共5页
Chinese Journal of Clinical Anatomy
基金
国家自然科学基金(81172555)
