摘要
为了解一氧化氮(NO)处理对番木瓜果实乙烯生物合成的影响,采用60μL/L NO熏蒸处理采收成熟度为果皮浅绿并微带黄色痕迹的番木瓜果实3 h,然后在20℃和相对湿度为85%条件下贮藏20 d。研究NO对番木瓜乙烯、1-氨基环丙烷-1-羧酸(ACC)、丙二酰-1-氨基环丙烷-1-羧酸(MACC)、ACC合成酶(ACS)和ACC氧化酶(ACO)及CpACS2和CpACO1基因表达的影响。结果表明,NO处理降低了番木瓜果实乙烯释放量、ACO的活性及CpACO1基因的表达,导致贮藏过程果实ACC和MACC的积累,但对ACS的活性及CpACS2基因的表达无显著抑制作用。
Effects of nitric oxide （NO） on ethylene biosynthesis in papaya fruit were investigated. Papaya fruit harvested at light green with some hint of yellow at the blossom end, were first fumigated with 60 υL/L NO for 3 h, then stored at 20℃ with 85% relative humidity （RH） for 20 days. Changes in the levels of ethylene, 1-aminocyclopropane-1-carboxylic acid （ACC）, 1- malonylaminoeyelopropane-l-earboxylie acid （MACC）, as well as the activities of ACC synthase （ACS）, ACC oxidase （ACO） and the expression of CpACS2 and CpAC01 genes were measured during papaya ripening. The results showed that NO treatment inhibited ethylene production, ACO activity and the expression of CpA CO1 gene, leading to the accumulation of ACC and MACC during storage. However, ACS activity and the expression of CpA CS2 gene were not suppressed significantly by NO treatment.
出处
《广东农业科学》
CAS
CSCD
北大核心
2013年第3期75-78,共4页
Guangdong Agricultural Sciences
基金
国家自然科学基金-广东省联合基金项目(U0631004)
广东省自然科学基金团队项目(06200670)
科技部支撑计划项目(2011BAD24B02-4)
