摘要
目的探讨不同精卵共孵育时间对小鼠体外受精受精率、氧化应激产生及胚胎质量的影响,及小鼠体外受精最短的精卵共孵育时间。方法分别比较ICR小鼠精卵共孵育30s、2min、10min、0.5h、2h、4h及18h的体外受精的受精率及囊胚形成率,并对精卵共孵育0h、2h及18h培养液的丙二醛浓度进行检测。结果各组获得卵细胞数分别为52、53、53、46、48、48及47,受精率分别为67.3%、81.1%、83.0%、87.0%、85.4%、81.3%及48.9%。精卵共孵育30s组的受精率显著低于0.5h及2h组(P<0.05),而18h组的受精率显著低于2min、10min、0.5h、2h及4h组(P<0.05)。2min组的受精率相比10min、0.5h、2h及4h组均无差异(P>0.05)。各组的囊胚形成率分别为82.9%、65.1%、70.5%、67.5%、75.6%、71.8%及65.2%,两两比较差异均无显著性(P>0.05)。精卵共孵育0h、2h及18h丙二醛浓度检测的均值分别为(1.06±0.21)×10-3mol/L、(1.44±0.22)×10-3mol/L及(2.00±0.21)×10-3mol/L,各组间两两比较差异均有显著性(P<0.05),丙二醛浓度随着精卵共孵育时间的缩短而降低。结论小鼠体外受精精卵共孵育时间缩短至2min不会降低受精率,同时随着孵育时间的缩短,产生的氧化应激也随之减少。
Objective To study the impact of gamete co-incubation time on fertilization rate, production of oxidative stress （OS） and embryo quality of mouse in vitro fertilization （IVF） , and to investigate the shortest gamete co- incubation time of mouse IVF. Methods Oocytes of ICR mouse were co-incubated with sperm for 30 seconds, 2 minutes, 10 minutes , 0. 5 hour, 2 hours, 4 hours and 18 hours, the fertilization rate and the blastocyst rate were analysed in each co-incubation group. In addition, the maleic dialdehyde（MDA） was assessed when gametes were co-incubated for 0 hour, 2 hours and 18 hours. Results Fifty-two, fifty-three, fifty- three, fourty-six, fourty-eight, fourty-eight and fourty-seven oocytes were inseminated with sperm in each group, and the fertilization rate were 67.3% , 81. 1% , 83.0% , 87.0% , 85.4% , 81.3% and 48.9% respectively. The fertilization rate of 30 seconds group was significantly lower than 0.5 hour and 2 hours groups（P 〈 0.05）. Moreover, the fertilization rate of 18 hours group was significantly lower than 2 minutes, 10rain, 0. 5 hour, 2 hours and 4 hours groups（P 〈0. 05）. No significant differences in fertilization rate were observed in 2 minutes, 10 minutes, O. 5 hour, 2 hours and 4 hours groups （P 〉 0.05 ）. In addition, the blastocyst rate were 82. 9%, 65. 1% , 70. 5% , 67.5% , 75. 6% , 71.8% and 65.2% , and no significant differences were observed in each group（P 〉0. 05）. The concentrations of MDA were （ 1.06± 0. 21 ） × 10 -3 mol/L, （ 1.44 ± 0. 22） × 10 -3 mol/L and （2. 00 ± 0. 21 ）× 10 -3 mol/L for 0 hour, 2 hours and 18 hours co-incubation, respectively. As the co-incubation time was reduced, the MDA levels were also significantly reduced （P 〈 0. 05 ）. Conclusion Reducing the gamete co-incubation time to 2 minutes will not affect the fertilization rate of mouse IVF. As the co-incubation time is reduced, the production of OS is also reduced.
出处
《解剖学报》
CAS
CSCD
北大核心
2013年第2期279-283,共5页
Acta Anatomica Sinica
基金
嘉兴市科技计划资助项目(2009BY7011)
嘉兴市政府重点科技创新团队基金资助项目(2011)
关键词
丙二醛
氧化应激
超短
体外受精
胚胎培养
辅助生殖技术
小鼠
Maleic dialdehyde
Oxidative stress
Ultrashort
In vitro fertilization
Embryo culture
Assisted reproductive technique
Mouse
