摘要
从中国人鼻咽癌细胞株 CNE2中克隆分离出的恶性转化基因 Tx,其基因长度为 1 6kb.在对其中 2 .8kb片段测序的基础上 ,对其中 Xho /Eco R 长度约 3.0 kb的片段 ( Tx3.0 )进一步进行了测序 ,并利用生物信息学技术分析认为 ,Tx3.0与人类免疫球蛋白 kappa( Igκ)轻链基因高度同源 ,并直接映射于 J区 .Tx3.0中除有编码免疫球蛋白 kappa链的 J2、J3、J4及 J5基因片段外 ,在各个片段间不仅有 TATA box、CAAT box和 Poly A等经典的调控序列 ,还有 NF- IL6的反应元件、某些转录因子的识别序列、以及核基质结合序列等 .据此以及 2 .8kb序列的分析结果 ,对 Tx3.0下游 1 .0 kb片段序列进行了预测 .对 Tx3.0基因片段的研究为进一步研究 Tx基因在鼻咽癌发病中的作用 ,提供了重要信息 .
Recently Cao Ya et al have reported that a new transforming gene,designated Tx gene,was isolated from human nasopharyngeal carcinoma(NPC) cell line CNE2.The previous data indicated that this gene was different from a number of known oncogene,and played a role in NPC carcinogenesis.The 2 8 kb Eco RⅠ/ Eco RⅠ fragment of Tx gene showed that it was highly homologous with Ig kappa constant region.Based on the restriction map of Tx gene,another Xho Ⅰ/ Eco RⅠ Tx 3 0 fragment was sequenced and analyzed by bioinformatics.This fragment contained Igκ J2,J3,J4 and J5 fragments,an N segment and several RSSs.Therefore,Tx3.0 was highly homologous with human Igκ gene and was in agreement with its J region.Besides classic canonical TATA boxes and CAAT boxes,Tx3.0 fragment also contained several NF IL6 binding sites,transcription factors binding sites and its 3′ terminal region contained a part of MAR.The identification of these elements and their precise localization in Tx3.0 are significant for further elucidating the role of Tx gene in carcinogenesis of NPC.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
2000年第5期602-605,共4页
Chinese Journal of Biochemistry and Molecular Biology
基金
中华医学基金!( CMB9665 5 )
国家自然科学资金重点项目!( 3 983 0 410 )资助
