摘要
在 4℃条件下 ,采用CaCl2 溶液浸泡褐藻裙带菜 (Undariapinnatifida)的叶状体 ,观察CaCl2 溶液的浓度和浸泡时间对裙带菜叶绿体提取率及其荧光特性的影响 .结果表明 ,裙带菜的叶状体经过CaCl2 溶液浸泡后 ,其叶绿体的提取率有明显地提高 .根据裙带菜叶绿体的提取率和室温荧光发射光谱的测定结果 ,认为最适的方法是采用 0 2mol/L的CaCl2 溶液浸泡 10min .在这种条件下 ,裙带菜叶绿体的提取率是传统制备方法的 5倍 .
Thalli from a brown alga Undaria pinnatifida were soaked by CaCl 2 solution with different concentration and time at 4℃, the effect of CaCl 2 solution on efficiency and influorescence emission spectra of chloroplasts were examined. The results show that the efficiency of collected chloroplasts is increased markedly after soaking in CaCl 2 solution. According to the results of collected efficiency and characteristic of influorescence emission spectra at room temperature of chloroplasts, it was suggested that soaking in the 0 2 mol/L CaCl 2 solution for 10 min is optimum. Under this condition, the efficiency of collected chloroplasts is as 5 fold as control group, and the characteristics of chloroplasts obtained by CaCl 2 soaking are similar to that of traditional method.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2000年第5期551-553,共3页
Progress In Biochemistry and Biophysics
关键词
褐藻
叶绿体
提取率
氯化钙浸泡
光合作用
brown algae, chloroplasts, collected efficiency, fluorescence emission spectra
