摘要
以黑龙江省大豆重茬 5年与正茬土壤和根系为主要研究对象 ,采用高效液相色谱法 ,研究土壤和根系浸提液中的酚酸物质的含量及其生物学效应 .结果表明 ,重茬土壤中对羟基苯甲酸和香草酸的含量 (1mol·L-1NaOH提取 )大于正茬土壤 ,且差异达到极显著水平 ,香草醛含量差异不显著 ;重茬大豆根系水提液中对羟基苯甲酸、香草酸、阿魏酸、香草醛、香豆素含量均高于正茬 .大豆连作条件下土壤多酚氧化酶活性高于正茬土壤 .重茬大豆根系水提液及在水培条件下外加对羟基苯甲酸对大豆幼苗生长发育有一定的抑制作用 ;酚酸物质加入土壤 1周后 ,对羟基苯甲酸、香草酸、香草醛、阿魏酸、苯甲酸、香豆素残留率分别为 10 .4%、15 .3 %、4.1%、2 .3 %、5 .0 %、17.5 % ;且外加酚酸浓度与土壤中真菌数量呈极显著指数相关 .
The contents of phenolic acids in the extracts of soil and roots of continuously cropped for five years and normally cropped soybean in Heilongjiang province and their biological effects were studied with HPLC method.The result showed that the contents of p hydroxy benzoic acid,vanillic acid(extracted by 1mol·L -1 NaOH) in continuous cropping soil were significant higher than that in normal cropping soil,and the content of vanillin had no significant difference in these soils.The contents of p hydroxybenzoic acid,vanillic acid,vanillin,ferulic acids,and coumarin of aqueous extracts of soyhean roots were higher in continuous cropping than in normal cropping soil.The activity of polyphenol oxidase in continuous cropping soil was higher than in normal cropping soil.Under conditions of water culture and adding exogenous p hydroxybenzoic acid,the aqueous extract of soybean roots inhibited the growth of soybean seedlings.After a week of exogenous phenolic acids addition,the residual rates of p hydroxybenzoic acid,vanillic acid,vanillin,benzoic acid,ferulic acids,and coumarin were 10.4%, 15.3%, 4.1%, 2.3%, 5.2%, 17.5%, respectively.There was a very significant exponential relationship between the addition amount of exogenous phenolic acids and the fungi population in soil.
出处
《应用生态学报》
CAS
CSCD
2000年第5期741-744,共4页
Chinese Journal of Applied Ecology
基金
国家"九五"重中之重招标资助项目!(95 0 1 0 5 2B0 3)
