摘要
以ｐＡＨＣ２０（含Ｂａｒ基因）和ｐＷＲＧ１５１５（含ＧＵＳ基因和潮霉素抗性基因）以及含Ｂａｒ基因和雪莲凝集素（ＧＮＡ）基因的ｐＣＡＭＢＩＡ３３００ ＲＧ为供体ＤＮＡ，选用水稻品系８７２０３、上农香糯及鄂宜１０５的成熟胚诱导出的愈伤组织及微不定芽为受体材料，分别采用基因枪和根癌农杆菌（ＬＢＡ４４０４，含ｐＡＭ４０４）导入法进行基因转化；经抗性筛选、ＧＵＳ检测和ＰＣＲ分析。结果表明，外源基因已通过基因枪法导入水稻鄂宜１０５，并获得再生植株；
Using pAHC20 (containing Bar gene), pWRG1515 (containing GUS gene and hygromycin phosphotransferase gene), and pCAMBIA3300 RG with Bar gene and snowdrop lectin (GNA) gene as donor DNA, the micro-adventitious shoots and the calli induced from mature embryos of Oryza sativa 87203. Eyil05. Shangnong aromatic glutinous rice as recipients were transformed with particle bombardment and Agrobacterium tumefaciens strain LBA4404 containing PAM404, respectively. After chosen with phosphinothricin and antibiotic, GUS detection and PCR analysis, The results showed that the foreign genes had been transformed microprojectile-mediated to Oryza sativa Eyil05, the regeneration plants were obtained, and, 5 transgenic calli of Oryza sativa Eyil05 were obtained with Agrobacterium-mediated transformation.
基金
上海市教委曙光计划资助项目!(96SG19)
关键词
水稻
抗除草剂
BAR基因
基因枪导入法
Oryza sativa
herbicide-resistance, Bar gene
microprojectile-mediated transformation, Agrobacterium
