摘要
首先利用凝胶阻滞电泳 ( electrophoretic mobility shift assay,EMSA )和免疫沉淀( immunoprecipitation,IP)方法观察两条 IL- 6信号转导途径—— JAK/STAT和 Ras/NF- IL- 6在人骨髓瘤细胞系 Sko- 0 0 7中的诱导激活状态 ;继而采用分别作用于两条途径的蛋白激酶抑制剂或激动剂以及转录因子的反义核酸与 IL- 6共同作用 Sko- 0 0 7细胞 ,观察一条途径激活信号上调 (或下调 )时 ,另外一条途径活化状态的变化 .结果显示 :1 .JAK/STAT和 Ras/NF- IL- 6信号转导途径都能够在 Sko- 0 0 7细胞中诱导激活 ;2 .与 JAK/STAT途径活化相关的某个酪氨酸磷酸化作用参与了Ras途径的诱导激活 ;Ras途径可通过蛋白激酶 MAPK( motigen- activated protein kinase)对 JAK/STAT途径的激活起正调控作用 .这说明在人骨髓瘤细胞 Sko- 0 0 7中两条 IL- 6信号途径可相互调控彼此的活化 .
Activation of two IL\|6 signal transduction pathways——Ras/NF IL 6 and JAK/STAT by IL 6 in a human multiple myeloma cell line (Sko\|007) was detected by electrophoretic mobility shift assay(EMSA) and immunoprecipitation(IP),respectively.Then the cells were treated by IL\|6 as well as the angonists or the antagonists specific for the protein kinases or the anti\|sense nucleic acids for the transcription factors in the two pathways.The results of EMSA and IP indicated that both of the two IL\|6 signal transduction pathways were activated by IL\|6 in Sko\|007 cells;some tyrosine phosphorylation effect related to JAK/STAT pathway was involved in the activation of Ras/NF\|IL\|6 pathway and Ras/NF\|IL\|6 pathway also gave a positive regulation on the activation of JAK/STAT pathway through the protein kinase\|MAPK.It is concluded that crossactivation may exist between the two IL\|6 signal transduction pathways in Sko\|007 cells.
出处
《中国生物化学与分子生物学报》
CSCD
2000年第5期655-659,共5页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家杰出青年科学基金!( 3 992 5 0 19)资助
