摘要
目的探讨在小鼠淋巴道转移潜能不同的肝癌细胞中,肝癌细胞增殖侵袭能力与膜联蛋白A7的亚细胞定位和亚型的表达。方法以小鼠腹水型肝癌高、低淋巴道转移潜能不同的细胞株Hca-F细胞和Hca-P细胞为研究对象,通过CCK8法检测细胞的增殖能力,Transwell小室检测细胞侵袭能力;Western blotting方法检测膜联蛋白A7在体外增殖侵袭能力不同的肿瘤细胞中的亚细胞定位和亚型的变化。结果发现Hca-F细胞无论是增殖能力还是侵袭能力都高于Hca-P细胞。膜联蛋白A7在Hca-F细胞和Hca-P细胞主要为47 kD亚型表达,而51 kD亚型只少量表达于细胞浆。47 kD亚型在Hca-F细胞的细胞浆、细胞膜、细胞器膜以及细胞骨架中的表达均明显高于Hca-P细胞,分别为(73%±7%)v(s49%±19%)、(36%±8%)v(s24%±9%)和(53%±7%)v(s40%±12%)(均P<0.05)。结论膜联蛋白A7的47 kDa亚型在细胞浆、细胞膜、细胞器膜和细胞骨架的定位表达,可能与肝癌细胞增殖侵袭能力密切相关。
[Objective] To study the cell proliferation and invasion activity in mouse hepatocarcinoma cell lines with different lymphatic metastatic potential and the location and isoform expression of Annexin A7. [Methods] Mouse hepatoearcinoma cell lines Hea-F with high lymphatic metatastie potential and Hea-P with low lymphatic metastatic potential were used. Cell proliferation was assessed by cell counting kit-8 （CCK8） assay. Cell invasion ability was analyzed by the Boyden-transwell assay. The location and isoform expression of annexin A7 were evaluated by subcellular fractionation kit and western blotting analysis. [Results] The perliferation and invasion ability were higher in Hca-F cell than those in Hca-P cell. 47 kD isoform was pre- sent in cytoplasm, membrane and cytoskeleton. And they were higher in Hca-F than those in Hca-P, where 51 kD was in the cytoplasm exclusively. [Conclusion] The location of annexin A7 47 kD isoform might at- tribute to tumor lymphatic metastasis.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2012年第35期9-13,共5页
China Journal of Modern Medicine
基金
国家自然科学基金(No:81071725)
教育部留学回国人员科研启动基金
