摘要
于 1 996年 8月在日本东京水产大学坂田试验场海岸岩礁地带采集齿突斜纹蟹(Plagusiadentipes)样品 ,参考果蝇 (Drosophilayakuba)与蚤状 氵蚤 (Daphniapulex)线粒体DNA中 1 2SrRNA基因片段序列进行了其引物设计、PCR扩增及序列测定 ,得到齿突斜纹蟹线粒体DNA中 1 2SrRNA基因片段的碱基序列为 447bp ,其中腺嘌呤 (A)、胸腺嘧啶 (T)、鸟嘌呤 (G)、胞嘧啶 (C)含量分别为 1 60bp ( 35 8% )、 1 62bp ( 36 2 % )、78bp ( 1 7 5% )、 47bp ( 1 0 5% ) ,与果蝇和蚤状 氵蚤 1 2SrRNA相同基因片段序列含量相似。
In August 1996, \%Plagusia dentipes\% was collected from rocky and weedy intertidal area near the Banda Marine Laboratory of Tokyo University of Fisheries, Chiba Prefecture, Japan The primers of 12S rRNA gene in mtDNA of \%Plagusia dentipes\% were designed by using the sequences of \%Drosophila yakuba\% and \%Daphnia pulex\% We amplified and sequenced the 447 base pairs that encode the 12S rRNA region of mitochondrial DNA The A, T, G, C contents of the sequences were 160 bp (35 8%),162 bp (36 2%), 78 bp(17 5%), 47 bp (10 5%) respectively, which are similar to the same region of the sequences of \%Drosophila yakuba\% or \%Daphnia pulex\%
出处
《大连水产学院学报》
CSCD
2000年第2期98-102,共5页
Journal of Dalian Fisheries University
基金
国家自然科学基金项目!(39970578)
