摘要
目的 :研究槲皮素对血管内皮细胞损伤的保护作用及机制。 方法 :实验分对照组、模型组和槲皮素组 ,用过氧化氢诱导血管内皮细胞损伤 ,四甲基偶氮唑蓝比色法检测细胞存活数量 ,放射免疫法测定细胞培养液中的内皮素及前列环素含量 ,用荧光法检测细胞内脂质过氧化产物丙二醛以评价脂质过氧化程度。 结果 :槲皮素 5、2 0、40、80和 10 0 μmol/ L 促进培养的正常血管内皮细胞增殖 ,增加前列环素生成 ,减少内皮素基础释放量 ;75 μmol/ L过氧化氢可损伤培养的血管内皮细胞 ;槲皮素 5、2 0和 80 μmol/ L 可抑制过氧化氢损伤的血管内皮细胞释放乳酸脱氢酶、内皮素及促进损伤的内皮细胞释放前列环素。 结论 :槲皮素具有保护血管内皮细胞损伤的作用 ,其机制可能与其抗脂质过氧化作用有关。
Objective:To evaluate the effects of quercetin on the injured vascular endothelial cells and its mechanism. Methods:Experiments were divided into control group,model group and Quercetin groups.Vascular endothelial cell (VECs) viability was assessed by methyl thiazolyl tetrazolium(MTT) assay.Radioimmunoassay (RIA) was used to assess the amount of endothelin (ET) and prostacyclin (PGI 2).Lipid peroxidation of VECs was monitored as malonaldehyde (MDA) with a fluorometric assay. Results:Quercetin increased the proliferation of normal VECs and the production of PGI 2 and inhibited the release of ET at the concentration of 5,20,40,80,100 μmol/L.Preincubation of VECs with 5,20,40,80 and 100 μmol/L of Quercetin for 24 h before H 2O 2 exposure significantly increased the cell viability and PGI 2 level,reduced lactate dehydrogenase (LDH) and ET release and MDA production. Conclusion:These results demonstrate that Quercetin can produce the protective action on H 2O 2 injured cultured VECs and its mechanism of action may be related to antioxidant effect of lipids.
出处
《中国循环杂志》
CSCD
北大核心
2000年第5期304-305,共2页
Chinese Circulation Journal
关键词
槲皮素
血管内皮细胞
细胞培养
动脉硬化
Quercetin
Vascular endothelial cell
Cultured cells
Hydrogen peroxide
