摘要
目的:探讨运动性免疫失衡对早期B细胞发育及调节体系的影响。方法:SPF级雄性SD大鼠128只,随机分成绝对安静组(AC)组,运动前安静组(A)、运动后即刻组(J)、运动后3H组(3H),进行6周递增负荷跑台运动,分别在第0、2、4、6周用四色流式细胞术检测大鼠早期immature B、mature B细胞群数量及凋亡情况。结果:0周:各组间immature B、mature B细胞群数量及凋亡情况均无显著性差异(P>0.05);2周,与A组相比,J组immature B、mature B数均呈显著性下降(P<0.05),3H组均显著性上升(P<0.05),而J组immature B细胞凋亡率呈显著性上升(P<0.05),mature B细胞凋亡率则呈显著性下降(P<0.05);4周:immature B细胞数在J组呈显著性下降(P<0.05),而mature B细胞数在3H组呈显著性上升(P<0.05),J组immature B细胞凋亡率呈显著性上升(P<0.05);6周:J组仅mature B细胞数呈显著性下降(P<0.05),仅immature B细胞凋亡率J组呈显著性上升(P<0.05)。结论:1)提示六周递增负荷运动中,机体根据运动需要,通过改变凋亡率,进而促成早期发育中B细胞的数量百分比的改变,以求尽可能维持免疫稳态,缓解运动性免疫失衡;2)联系前期对Pro B,Pre B细胞数量及凋亡率的影响,本研究提示随训练时间的延长,运动负荷的增强,运动应激对mature B凋亡率的影响却是渐趋减弱的;3)结合前期研究成果,提示6周递增负荷运动可能通过代偿机制促使存活的Pre B细胞更多的分化成为mature B淋巴细胞,以适应机体大量耗能的需要。
Objective ： The purpose of this study is to explore the effects of exercise - induced immune imbalance on the development and regulation system of early precursor B lymphocytes in rats. Meth- ods：128 Sprague - Dawley rats were randomly assigned to groups of absolute control（AC）, control （A） , immediateness （J） and 3 hours after exercises （ 3 H）, respectively. The percentage and apop- tosis of immature B, mature B cell were measured by flow cytometry at the end of 0 week, 2nd,4th and 6th week. Results：WK 0：The percentage of immature B and mature B cell had no significant changes at WK0 （J）,WK0 （3H） co with WK0 （A）. WK 2：The percentage of immature B and mature B cells at WK2 （J） had shown a significant decrease （ P 〈 0.05 ） compared with WK2 （A）group. The apoptosis rate of immature B cells and mature B cells at WK2 （J） showed a significant in- crease （ P 〈 0.05 ） and decrease （ P 〈 0.05 ）, respectively. WK 4 ： The percentage of immature B at WK4 （J） significantly decreased （ P 〈 0.05 ） compared with WK4 （A） group. The percentage of ma- ture B at WK 4 （ 3 H） and the apoptosis rate of immature B cells at WK4 （J） significantly increased （ P 〈 0.05 ）. WK6 ： The percentage of mature B at WK6 （J） significantly decreased （ P 〈 0.05 ） compared with WK6（A） group. The apoptosis rate of immature B cells at WK6（J） were significant- ly increased （ P 〈 O. 05 ）. Conclusion ： 1 ） It suggested that immature B and mature B cell mass changed by increasing the apoptosis rate according to exercise intensity to maintain immune homeo- stasis as much as possible to ease the exercise- induced immune imbalance;2）As training time in- creases, the effects of incremental exercise showed gradually weakened effect on the apoptosis rate of mature B cells ;3 ）Combined with the preliminary research, it is supposed that long -term exercise induced compensatory mechanisms to promote more surviving Pre B cells to differentiate to mature B lymphocytes, and to adapt to the needs of the body to mobilize more energy.
出处
《山东体育学院学报》
北大核心
2013年第1期60-65,共6页
Journal of Shandong Sport University
基金
山东体育学院重点课题(111101)
关键词
B淋巴细胞
发育
调节体系
细胞凋亡
骨髓
运动性免疫失衡
B lymphocytes
development
regulation system
apoptosis
bone marrow
exercise - in-duced immune imbalance
