摘要
目的比较不同方法提取川芎中藁本内酯和阿魏酸及香附中α-香附酮的溶出度,确定最佳提取方法。方法建立测定藁本内酯、阿魏酸及α-香附酮的高效液相色谱法,测定川芎和香附先醚后醇、醇渗滤和水煎煮3种方法提取物中的指标成分的含量。结果川芎和香附先石油醚后75%乙醇提取的总有效成分含量最高。结论采用分部提取再合并的方法,可提高川芎和香附有效成分的溶出度和提取率,减少不稳定成分的损失。
Objective To compare different extractioin methods for the dissolution of the ligustilide and ferulic acid in Ligusticum walliehii and α-cyperone in Rhizoma cyperi. Methods An HPLC method was established for the determination of ligustilide, ferulic acid and α-cyperone. The content of these index components were extracted via three methods, including alco- hol followed by ether, alcohol percolation, and water boiling. Results The total active constituents from the two Chinese medi- cines had the highest yield from petroleum ether extraction followed by 75% ethanol. Conclusion The combination of division and remerging extraction approach improves the dissolution and extraction rate of active ingredients as well as reduces the loss of unstable components.
出处
《医药导报》
CAS
北大核心
2013年第3期350-353,共4页
Herald of Medicine
基金
国家自然科学基金资助项目(81102836)
关键词
川芎
香附
藁本内酯
阿魏酸
Α-香附酮
Ligusticum wallichii
Rhizoma cyperi
Ligustilide
Ferulic acid
α-cyperone
