摘要
目的探讨参杞强精胶囊对奥硝唑致弱精子症大鼠精子质量的影响及其机制。方法以奥硝唑400 mg.kg-1.d-1灌胃建立大鼠弱精子症模型。分别灌胃给予参杞强精胶囊低、中、高剂量(400,800,1 600 mg.kg-1.d-1)。检测用药后大鼠精子活动力、精子活率、精子计数、附睾丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果与正常对照组相比,模型组精子活动力显著降低(P<0.01)、附睾MDA含量明显升高(P<0.01)、SOD活性明显降低(P<0.01)。与模型组相比,参杞强精胶囊中、高剂量组精子活动力明显增强(P<0.05或P<0.01),低剂量组虽有改善但差异无统计学意义;中、高剂量组MDA含量有明显改变(P<0.01),低、中、高剂量组SOD活力提高(P<0.05或P<0.01)。结论参杞强精胶囊能较好改善奥硝唑致弱精子症大鼠的精子活动力,可能与其调节抗氧化机制、降低氧化应激损伤有关。
Objective To investigate the effect of shenqi qiangfing capsule on the sperm quality in ornidazole-induced asthenospermic rats. Methods The rat asthenospermia model was established by intragastric administration of ornidazole at the dose of 400 mg· kg-1 · d-1. Animals were administrated with low, middle and high doses （400,800,1 600 mg · kg-1 ·d-1 ） of shenqi qiangfing capsules. The sperm motility, viability, and quantity as well as superoxide dismutase （SOD） and malondialde- hyde （MDA） levels in epididymis were measured. Results Compared with the normal control,sperm motility was significantly decreased （P〈0.01）, MDA content in epididymis was significantly increased （P〈0.01）, and the SOD activity was significantly reduced （P〈0.01） in the model control group. In the mid and high dose group, the sperm motility was increased significantly （P〈0.05 and P〈0.01, respectively）, and the MDA level was decreased significantly （P〈0.01） in comparison with the model control. The SOD activity was enhanced in all three treatment groups （P〈0.05 or P〈0.01 ）. Conclusion Shenqi qiangjing capsule can improve sperm motility of the male rats with ornidazole-induced asthenospermia, which might be related to modulation of the oxidative stress injury.
出处
《医药导报》
CAS
北大核心
2013年第3期278-281,共4页
Herald of Medicine
基金
广西壮族自治区卫生厅中医药医院制剂类课题(GZYZ-10-05)
广西壮族自治区中医药管理局中医药科技专项课题(GZYZ1101)
