摘要
目的探讨柚皮苷对心肌缺血-再灌注损伤的保护作用及其机制。方法 50只雄性SD大鼠随机分为5组,即假手术组,缺血-再灌注模型组,柚皮苷低、中、高剂量组,每组10只。建立心肌缺血-再灌注损伤模型,假手术组完成模型全部操作,只穿线不结扎。柚皮苷3个剂量组分别以相应剂量灌胃1次,连续给药7 d。于末次灌胃60 min后行缺血30 min,再灌注120 min。缺血-再灌注模型组给予等容量0.9%氯化钠溶液灌胃,其他处理同上。于再灌注结束后取心肌组织测定谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)和血清中肌酸激酶(CK)、乳酸脱氢酶(LDH)、过氧化氢酶(CAT)活性,心肌组织中丙二醛(MDA)含量。结果与模型组相比,柚皮苷低、中、高剂量组大鼠血清中CK[(3.44±0.06),(2.71±0.08),(2.21±0.08)U.mL-1]和LDH[(3 780.88±248.44),(3 339.58±234.28),(2 693.57±130.59)U.mL-1]活性和心肌组织中MDA[(4.46±0.27),(3.81±0.21),(2.92±0.17)nmol.mg-1]含量均降低(P<0.05或P<0.01),血清中CAT[(19.77±0.98),(22.83±0.83),(24.47±0.63)U.mL-1]和心肌组织GSH-Px[(25.36±0.84),(31.40±1.10),(39.08±1.16)U.mg-1]和SOD[(92.05±3.08),(112.59±4.83),(129.88±5.82)U.mg-1]活性均升高(P<0.01)。结论柚皮苷对缺血-再灌注损伤心肌具有保护作用,可能与其抗氧化活性和减轻脂质过氧化有关。
Objective To investigate the protective effect of naringin on myocardial ischemia/reperfusion injury in rats and its possible mechanism. Methods Fifty male SD rats were randomly divided into five groups： sham operation group, is- chemia reperfusion injury model group, and groups of naringin at low, middle and high doses, each containing 10 rats. Myocardi- al ischemia reperfusion injury was inflicted and the sham group was only threaded without ligation after completing all the opera- tion. Three naringin groups were intragastrically administrated with corresponding doses of naringin once daily for 7 successive days. Sixty minutes after the last dose, animals were ligated for 30 minutes followed by 120 minutes of reperfusion. The model control was intragastrically administrated with the same volume of physiological saline and operated as other groups. The activities of glutathion peroxidase （GSH-Px） , superoxide dismutase （SOD）and the content of melondialdehyde （MDA） were detected in the myocardial tissue, and the activities of creatine kinase（ CK）, lactate dehydrogenase （LDH）, catalase （CAT） in blood serum were also analyzed by the end of reperfusion. Results Compared with the model group, the activities of CK （3.44±0.06, 2.71±0.08 ,and 2.21±0.08 U · mL-l ） and LDH （3 780.88±248.44,3 339.58±234.28 ,and 2 693.57±130.59 U · mL-1 ） in blood serum and the content of MDA （4.46±0.27, 3.81±0.21 , and 2.92±0.17 nmol ·mg -1 ） in myocardial tissue were de- creased in the low,middle and high dose groups of naringin （P〈0.05 and P〈0.01 ）. On the contrary, the activities of the CAT （ 19.77±0.98,22.83±0.83 ,and 24.47±0.63 U ·mL-1 ） in blood serum and GSH-Px （25.36±0.84,31.40±1.10 ,and 39.08± 1.16 U · mg-1 ） and SOD （92.05±3.08,112.59±4.83,and 129.88±5.82 U ·mg-1 ） in myocardial tissue were increased in these groups （P〈0.01）. Conclusion Naringin has protective effects on myocardial ischemia/reperfusion injury, the mecha- nism of which may be correlated with the antioxidant activity and reducing the lipid peroxidation.
出处
《医药导报》
CAS
北大核心
2013年第3期275-277,共3页
Herald of Medicine
基金
重庆市万州区科技计划项目(200903022)
关键词
柚皮苷
心肌缺血
损伤
缺血-再灌注
超氧化物歧化酶
丙二醛
Naringin
Myocardial ischemia
Injury, ischemia/reperfusion
Superoxide dismutase
Malondialdehyde
