期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

免疫磁珠法分离并鉴定前列腺癌类干细胞 被引量：2

Isolation and Identification of Prostate Cancer Stem-Like Cells by Magnetic Active Cell Sorting
暂未订购
导出
摘要 目的利用免疫磁珠法分选并鉴定人前列腺癌类干细胞。方法利用免疫磁珠法从人前列腺癌细胞株PC3中分选CD133+/CD44+细胞,通过免疫组化、流式细胞术、CCK8、平板克隆形成实验及裸鼠成瘤实验鉴定其表面标志物表达情况及肿瘤干细胞特性。结果经免疫磁珠法分选所得CD133+/CD44+细胞比例为0.50%;免疫组化及流式显示其CD44/CD133/整合素α2β1均高表达;其细胞增殖和克隆形成率高于PC3细胞;以低密度注射时,其裸鼠体内成瘤率明显高于PC3细胞。结论利用免疫磁珠法可从人前列腺癌细胞株PC3中高效的分选出具有肿瘤干细胞生物学特性的前列腺癌类干细胞。 Objective To isolate and identify the prostate cancer stem-like cells（CSCs） by magnetic active cell sorting（MACS）.Methods CD133＋/CD44＋ cells were selected from PC3 human prostatic cancer cells lines by MACS,and they were identified as CSCs by immunohistochemistry,flow-cytometric analysis,CCK8,colony-forming assay and tumorigenicity assay.Results Sorted cells showed higher proportion of CD133＋/CD44＋ cells,integrinα2β1＋ cells and enhanced tumorigenic potential in vitro and in vivo when seeded at a low density.Conclusion MACS was a simple and effective method to gain prostate CSCs from PC3 cell lines.
作者 秦亮 陈安民 郭风劲 宫晨 卞峰 姚斌 廖晖
机构地区 华中科技大学同济医学院附属同济医院骨科
出处 《中华全科医学》 2013年第3期336-337,389,F0003,共4页 Chinese Journal of General Practice
基金 教育部新教师基金项目(200804871051) 湖北省卫生厅青年人才基金(QJX2010-5)
关键词 肿瘤干细胞 前列腺癌 免疫磁珠分选 Cancer stem cells Prostate cancer Magnetic active cell sorting
分类号 R737.25 [医药卫生—肿瘤] R730.21 [医药卫生—肿瘤]
  • 相关文献

参考文献11

  • 1Marx J. Mutant stem cells may seed cancer [J]. Science, 2003,301 (5638) : 1308-1310.
  • 2Singh SK, Clarke ID, Terasaki M, et al. Identification of a cancer stem cell in human brain tumors [ J ]. Cancer Res, 2003,63 ( 18 ) : 5821- 5828.
  • 3Ponti D, Costa A, Zaffaroni N, et al. Isolation and in vitro propagation of tumorigenic breast cancer cells with stern/progenitor cell properties [ J ]. Cancer Res ,2005,65 ( 13 ) :5506-5511.
  • 4Collins AT, Berry PA, Hyde C, et al. Prospective identification of tumorigenic prostate cancer stem ceils [ J ]. Cancer Res, 2005,65 (23) : 10946-10951.
  • 5孙颖浩.我国前列腺癌的研究现状[J].中华泌尿外科杂志,2004,25(2):77-80. 被引量：356
  • 6Tang DG, Patrawala L, Calhoun T, et al. Prostate cancer stem/progenitor ceils :identification, characterization, and implications [ J ]. Mol Carcinog,2007,46 ( 1 ) : 1-14.
  • 7Lawson DA, Xin L, Lukacs RU, et al. Isolation and functional characterization of murine prostate stem cells [ J ]. Proc Natl Acad Sci USA, 2007,104( 1 ) :151-186.
  • 8Patrawala L, Calhoun T, Schneider-Broussard R, et al. Highly purified CD44^+ prostate cancer cells from xenograft human tumors are enriched in tumorigenie and metastatic progenitor cells[J]. Oncogene ,2006,25 (12) :1696-1708.
  • 9Shepherd C J, Rizzo S, Ledaki I, et al. Expression profiling of CD133^+ and CD133 - epithelial cells from human prostate [ J ]. Prostate, 2008, 68(9):1007-1024.
  • 10李曾,王德林,蓝建华,杨宗珂.人前列腺癌细胞株PC-3中类肿瘤干细胞的培养分离和初步鉴定[J].解放军医学杂志,2009,34(11):1348-1352. 被引量：4

二级参考文献10

  • 1欧阳振,王栓科,康学文,王翠芳,汪静,郭洪章.人骨肉瘤细胞株U2-OS中分离并鉴定肿瘤干细胞[J].中国组织工程研究与临床康复,2007,11(24):4706-4709. 被引量：6
  • 2Ponti D, Losta A, Zaffaroni N, et al. Isolation and invitro propagation of tumorigenic breast cancer cells with stem/progenitor cell properties. Cancer Res, 2005, 65(13): 5506.
  • 3Singh SK, Clarke ID, Terasaki M, et al. Identification of a cancer stem cell in human brain tumors. Cancer Res, 2003, 63(18): 5821.
  • 4Rizzo S, Attand G, Hudson DL. Prostate epithelial stem cells. Cell Prolif, 2005, 38(8): 363.
  • 5Collins AT, Maitland NJ. Prostate cancer stem cells. Eur J Cancer, 2006, 42(9): 1213.
  • 6Abroad K. Small subsets of cells initiate brain tumours. Lancet Oncol, 2005, 6(1): 9.
  • 7Collins AT, Habib FK, Maitland NJ, et al. Identification and isola tion of human prostate epithelial stem cells based on α2β1-integrin expression. Cell Sci, 2001, 114(21): 3865.
  • 8Garin D, Graig N, Shona H, et al. CD133, a novel marker for human prostatic epithelial stem cells. Cell Sci, 2004, 117(16): 3539.
  • 9王彬,杨辉,尹昌林,吕胜青,刘海鹏.恶性胶质瘤细胞株U251中肿瘤干细胞的分离、培养及鉴定[J].中国临床神经科学,2007,15(6):638-642. 被引量：10
  • 10董强刚,姚明,耿沁,周瑾,闫明霞.人肺腺癌干细胞的分离及鉴定[J].肿瘤,2008,28(1):1-7. 被引量：42

共引文献357

同被引文献25

  • 1Visvader E,Geoffrey J.Lindeman.Cancer stem cells:current status and evolving complexities[J].Cell stem cell,2012,10(6):717-728.
  • 2Lee SO,Ma Z,Yeh CR,et al.New therapy targeting differential androgen receptor signaling in prostate cancer stem/progenitor vs.nonstem/progenitor cells[J].J Mol Cell Biol,2013,5(1):14-26.
  • 3Bonham M,Arnold H,Montgomery B,et al.Molecular Effects of the Herbal Compound PC-SPES Identification of Activity Pathways in Prostate Carcinoma[J].Cancer Res,2002,62(14):3920-3924.
  • 4Mimeault M,Mehta PP,Hauke R,et al.Functions of normal and malignant prostatic stem/progenitor cells in tissue regeneration and cancer progression and novel targeting therapies[J].Endocrine Reviews,2008,29(2):234-252.
  • 5Baumann M,Krause M,Hill R.Exploring the role of cancer stem cells in radioresistance[J].Nature Reviews Cancer,2008,8(7):545-554.
  • 6Williams K,Motiani K,Giridhar PV,et al.CD44integrates signaling in normal stem cell,cancer stem cell and(pre)metastatic niches[J].Exp Biol Med(Maywood),2013,238(3):324-338.
  • 7Trapasso S,Allegra E.Role of CD44as a marker of cancer stem cells in head and neck cancer[J].Biologics,2012(6):379-383.
  • 8Patrawala L,Calhoun T,Schneider-Broussard R,et al.Highly purified CD44+prostate cancer cells from xenograft human tumors are enriched in tumorigenic and metastatic progenitor cells[J].Oncogene,2006,25(12):1696-1708.
  • 9Richardson GD,Robson CN,Lang SH,et al.CD133,a novel marker for human prostatic epithelial stem cells[J].J Cell Sci,2004,117(16):3539-3545.
  • 10Collins AT,Berry PA,Hyde C,et al.Prospective identification of tumorigenic prostate cancer stem cells[J].Cancer Res,2005,65(23):10946-10951.

引证文献2

二级引证文献12

中华全科医学
2013年 第3期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部