摘要
目的检测miR-373在骨肉瘤细胞中表达情况,并探讨其对细胞增殖、侵袭和迁移的影响。方法体外培养人骨肉瘤细胞株SJSA-1及人骨肉瘤细胞hFOB 1.19,将SJSA-1随机分为4组,采用FuGENE®HD转染试剂转染细胞,4组细胞分别为空白对照组(CK组)、阴性对照组(NC组)、miR-373过表达质粒组(miR-373 mimic组)及miR-373抑制质粒组(miR-373 inhibitor组),转染48 h后,qRT-PCR法检测miR-373的相对表达量,CCK-8法检测细胞增殖情况,Transwell法检测细胞侵袭与迁移的情况。结果SJSA-1细胞miR-373相对表达量明显高于hFOB 1.19细胞(P<0.05);与CK组、NC组比较,miR-373 mimic组细胞miR-373相对表达量明显升高(P<0.05),miR-373 inhibitor组细胞miR-373相对表达量明显降低(P<0.05);miR-373 mimic组培养24、36、48 h的OD值均明显高于CK组、NC组(P<0.05),miR-373 inhibitor组培养24、36、48 h的OD值均明显低于CK组、NC组(P<0.05);miR-373 mimic组细胞迁移数目明显多于CK组、NC组(P<0.05),miR-373 inhibitor组细胞迁移数目明显少于CK组、NC组(P<0.05);miR-373 mimic组细胞侵袭数目明显多于CK组、NC组(P<0.05),miR-373 inhibitor组细胞侵袭数目明显少于CK组、NC组(P<0.05)。结论骨肉瘤细胞中miR-373表达上调,miR-373过表达可促进骨肉瘤细胞的增殖、侵袭和迁移,miR-373低表达可抑制骨肉瘤细胞的增殖、侵袭和迁移,有望成为临床骨肉瘤诊断和治疗的重要靶点。
Objective To detect the expression of miR-373 in osteosarcoma cells and explore its effects on cell proliferation,invasion,and migration.Methods Human osteosarcoma cell line SJSA-1 and human osteoblast hFOB 1.19 cultured in vitro.Human osteosarcoma cell line SJSA-1 were randomly divided into four groups:blank control group(CK group),negative control group(NC group),miR-373 over-expressed plasmid group(miR-373 mimic group)and miR-373 inhibited plasmid group(miR-373 inhibitor group).Cells were transfected with FuGENE®HD transfection reagent.After 48 hours of transfection,the relative expression of miR-373 was detected by quantitative real-time polymerase chain reaction(qRT-PCR),cell proliferation by cell counting kit-8(CCK-8)and cell invasion and migration by Transwell.Results The relative expression of miR-373 in SJSA-1 cells was significantly higher than that in hFOB-1.19 cells(P<0.05);compared with CK group and NC group,the relative expression of miR-373 in the miR-373 mimic group increased significantly(P<0.05),and the relative expression of miR-373 in the miR-373 inhibitor group was significantly lower than that in the control group(P<0.05);optical density(OD)value of miR-373 mimic group at 24 h,36 h,and 48 h were significantly higher than that of CK group and NC group(P<0.05),and OD value of miR-373 inhibitor group at 24 h,36 h,and 48 h were significantly lower than that of CK group and NC group(P<0.05);the number of cell migration in the miR-373 mimic group was significantly higher than that in the CK group and NC group(P<0.05),and the number of cell migration in the miR-373 inhibitor group was significantly lower than that in CK group and NC group(P<0.05);the number of cell migration in the miR-373 mimic group was significantly higher than that in the CK group and NC group(P<0.05),and the number of cell migration in the miR-373 inhibitor group was significantly lower than that in CK group and NC group(P<0.05).Conclusions The expression of miR-373 is up-regulated in osteosarcoma.Overexpression of miR-373 can promote the proliferation,invasion,and migration of osteosarcoma cells,while the low expression of miR-373 can inhibit the proliferation,invasion,and migration of osteosarcoma cells,which may be an important target for the diagnosis and treatment of osteosarcoma.
作者
谭晓谦
梅海波
刘昆
唐进
伍江雁
朱光辉
叶卫华
Tan Xiaoqian;Mei Haibo;Liu Kun;Tang Jin;Wu Jiangyan;Zhu Guanghui;Ye Weihua(Department of Orthopedics,Hunan Children′s Hospital,Changsha 410007,China)
出处
《中国医师杂志》
CAS
2020年第1期91-95,共5页
Journal of Chinese Physician
