摘要
采用直链淀粉Chiralpak AD-H与纤维素Chiralcel OZ-H两种手性柱建立了13种黄酮类化合物对映体的正相高效液相色谱拆分方法。考察了流动相比例、三氟乙酸(TFA)的添加及醇的种类对手性识别的影响。以正己烷-异丙醇(乙醇)-三氟乙酸(75∶25∶0.1)为流动相,于流速为0.5 mL.min-1,柱温为35℃,检测波长为290 nm条件下,在直链淀粉Chiralpak AD-H与纤维素Chiralcel OZ-H两种手性柱上成功拆分了13种黄酮类化合物对映体。研究表明,直链淀粉Chiralpak AD-H与纤维素Chiralcel OZ-H两种手性柱在拆分手性黄酮类化合物及结构相似物上具有极大的潜力。
An HPLC method was developed for the enantioseparation of 13 flavonoids on chiral col- umns of Chiralpak AD - H （250 mm×4.6 mm, 5 μm） and Chiralcel OZ - H （250 mm × 4. 6 mm, 5 μm）. The influences of type and percentage of alcohol modifier on the chiral recognition were examined. Effects of trifluoroacetic acid （TFA） in the mobile phase were also studied. The optimized chromatographic conditions for 13 racemic flavonoids were as follows： mobile phas： n-hexane-isopropanol （ethanol） -TFA（75 ： 25 ： 0. 1）, flow rate： O. 5 mL·min-1, column temperature： 35 ℃, UV detection wavelength： 290 nm. Under the optimal conditions, 13 racemic flavonoids were successfully enantioseparated at the baseline on Chi This study illustrated the potentials of Chiralpak ralpak AD -H and Chiralcel OZ - H chiral columns AD-H and Chiralcel OZ-H chiral columns in the chiral separation of flavonoids and their analogues.
出处
《分析测试学报》
CAS
CSCD
北大核心
2013年第3期346-350,共5页
Journal of Instrumental Analysis
基金
科技部创新方法工作专项资助项目(2011IM030200)
