摘要
目的 :从鸡蛋中提取特异性抗体 ,鉴定抗体性质并分析其蛋白质含量 ,以探索一种制备被动免疫防龋抗体的制备方法。方法 :变形链球菌GTF高表达株S .mutansB2 9- 33及茸毛链球菌S .sobrinus 6 715免疫母鸡 ,采用硫酸铵沉淀法提取鸡蛋黄抗体 ,SDS -PAGE凝胶扫描鉴定抗体性质 ,分析其纯度并用分光光度法测蛋白质含量。结果 :用硫酸铵沉淀法可提取出鸡蛋黄抗体 ,其性质为IgG ,蛋白质含量约为 7.0 0mg/ml。结论 :鸡蛋黄抗体含量高 ,作为被动免疫抗体的载体有较好的应用前景。
Objective:The specific antibody was isolated from egg yolk in order to find a new method for passive immunization against dental caries. Methods:50 hens were divided into two groups and were injected with S.mutans B29-33 and S.sobrinus 6715 whole cells respectively. Egg yolk antibody IgY were isolated by sodium sulfate precipitating method and identified by SDS-PAGE. After electrophoresis the gel was scanned by chromatographic scanner and the protein content was evaluated by absorption spectrometry. Results: SDS-PAGE and gel scanner revealed the substance isolated from egg yolk was IgG. The protein content was 7.00mg/ml. Conclusion:Egg yolk antibody was isolated conveniently and the protein content was high. So it can be use as a suitable source of antibody for passive immunization.
出处
《口腔医学纵横》
CSCD
2000年第3期193-194,共2页
Journal of Comprehensive Stomatology
基金
scienceandtechnologykeyitemofHubeiprovince !(Number 982p1 60 2 )
关键词
鸡蛋黄
抗体
变形链球菌
制备
Egg yolk
Antibody
Streptococcus mutans
Purification
