摘要
为了揭示变形链球菌葡糖基转移酶（ｇｌｕｃｏｓｙｌｔｒａｎｓｆｅｒａｓｅ，ＧＴａｓｅ）在变链菌粘附中的作用，通过扫描电镜观察了其缺陷突变株对玻片的粘附。结果显示，在蔗糖存在的条件下，变链菌ＭＴ８１４８及其ＧＴａｓｅ缺陷株表现出不同的粘附模式。提出了细胞吸附型ＧＴａｓｅ（ＣＡＧＴａｓｅ）与细菌间的聚集有密切关系的观点，认为这种聚集的紧密程度高于由细胞外ＧＴａｓｅ（ＣＦＧＴａｓｅ）所介导的聚集。而ＣＦＧＴａｓｅ所合成的葡聚糖可能与细菌间某种有序的粘接有关。本研究结果对深入认识变链菌的致龋机理有参考价值。
The ultrastructure of adherence of Streptococcus mutans MT8148 and its glucosyltransferase deficient mutants were compared by Scanning Electronic Microscope. Four patterns of adherence of tested strains in the presence of sucrose were observed. These results suggest that the cell associated GTase is responsible for firm aggregation of cells. However, cell free GTase is also important in aggregation between cells.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
1997年第2期102-104,共3页
Chinese Journal of Stomatology
基金
国家自然科学基金
关键词
变形链球菌
GTF
细菌粘附
龋齿
Streptococcus mutans Bacterial adhesion Dental caries, enzymology
