摘要
目的对2010～2012年德州市11县市区1 739例手足口病病例进行病原谱分析。方法采用荧光定量PCR方法对手足口病病例粪便标本进行肠道病毒通用型、EV71型和Cox A16型核酸检测。结果 1 739例手足口病病例肠道病毒通用型核酸阳性率64.29%,其中EV71阳性率为25.24%,Cox A16阳性率为22.31%,其他肠道病毒阳性率为16.73%;2010年病原以Cox A16为主,2011年病原以EV71为主,2012年病原以Cox A16为主,且有其他待定型别肠道病毒流行,检出率高于Cox A16;不同县市区之间病原体类型的阳性率差异有统计学意义(χ2=308.629,P<0.01);2～3岁组发病率最高,<1岁组病例数最少;发病时间主要集中在每年的4～7月,5月份病例数最多;9例重症病例中6例由EV71引起,1例由Cox A16引起,其他2例肠道病毒检测为阴性。结论手足口病病原谱复杂,具有动态变化特征。应加强手足口病监测,在发病高峰到来之前提前做好各项防控工作。
Objective To analyze the pathogenic spectrum in 1 739 cases of hand, foot, and mouth disease （HFMD） from 11 counties of Dezhou from 2010 to 2012. Methods Fluorescent quantitation PCR was used to test stool specimens for enterovirus, enterovirus 71 （EV71）, and Cox A16. Results Of 1 739 cases of HFMD, 64.29% tested positive for enterovirus, 25.24 % tested positive for EV71, 22.31% tested positive for Cox A16, and 16.73% tested positive for oth-er enteroviruses. Cox A16 was the main pathogen in 2010 while EV71 was in 2011 ; Cox A16 was the main pathogen in 2012 and other enteroviruses （not EVT1 or Cox A16） were not prevalent. Positivity for Cox 16 was highest, and this rate differed significantly （X^2= 308. 629, P〈0.01） from the rate of positivity for similar pathogens in other counties. Inci-dence was highest among children ages 2-3 years and lowest among children under 1 year of age. Incidence was highest from April to July and peaked in May： of 9 cases of severe HFMD, 6 were caused by EV71, 1 was caused by Cox A16, and the remaining 2 were negative for an enterovirus. Conclusion The pathogenic spectrum of HFMD is complex and dynamic, so monitoring of the prevalence of HFMD should be enhanced and prevention and control should be implemented before incidence peaks.
出处
《中国病原生物学杂志》
CSCD
北大核心
2013年第2期131-134,122,共5页
Journal of Pathogen Biology
