摘要
目的 探讨三氯乙烯及其代谢产物三氯乙酸、三氯乙醇及水合三氯乙醛对豚鼠的皮肤致敏作用。方法 按豚鼠最大值试验 (GPMT)法 ,用三氯乙烯、三氯乙酸、三氯乙醇及水合三氯乙醛分别对白化豚鼠进行皮内和涂皮结合法致敏试验 ,并设阳性对照 (二硝基氯苯 )、溶剂对照 (橄榄油 )和福氏完全佐剂 (FCA)对照 ,观察各组动物皮肤的红斑和水肿等情况 ,求出致敏率和平均反应值。结果 三氯乙烯、三氯乙酸和二硝基氯苯的致敏率分别为 71 4%、5 8 3 %和10 0 % ,平均反应值分别为 2 3、1 1和 6 0。三氯乙醇和水合三氯乙醛的致敏率均为 0。结论 三氯乙烯是强度致敏物 ;三氯乙酸是中度致敏物 ;三氯乙醇及水合三氯乙醛未见致敏作用。
Objective The contact allergenic activity of trichloroethylene and its three metabolites trichloroacetic acid, trichloroethanol, chloral hydrate were studied. Methods Using a modified guinea pig maximization test(GPMT), the skin sensitization induced by (edema and erythema) trichloroethylene, trichloroacetic acid, trichloroethanol, chloral hydrate and 2,4 dinitrochloro benzene respectively were observed. The allergenic rates and mean response score were calculated. Results The allergenic rates of trichloroethylene, trichloroacetic acid and 2,4 dinitrochloro benzene were shown to be 71.4%, 58.3% and 100% respectively, whereas that of both trichloroethanol and chloral hydrate were 0%. The mean response scores of trichloroethylene, trichloroacetic acid, 2,4 dinitrochloro benzene were 2.3, 1.1, 6.0 respectively. Conclusion Trichloroethylene appeared to be a strong allergen and trichloroacetic acid to be a moderate one.
出处
《中国职业医学》
CAS
2000年第5期6-8,共3页
China Occupational Medicine
基金
广东省重点科技项目部分工作!(编号 962 2 0 56 - 0 5)
关键词
三氯乙烯
三氯乙酸
三氯乙醇
皮肤致敏试验
GPMT
Trichloroethylene, trichloroacetic acid, trichloroethanol, chloral hydrate, guinea pig maxmization test
