摘要
以双亚7号成熟种子为材料,利用秋水仙碱浸泡亚麻种子后进行无菌培养来诱导多倍体。方法是将种子消毒后在培养皿中萌动处理后,利用秋水仙碱处理来诱导多倍体。结果表明:种子在水中萌动时间为36 h,秋水仙碱溶液处理浓度为0.075%,处理时间为24 h,变异率为40.7%。通过对外部形态的观察发现四倍体的茎比二倍体的粗壮,叶片较二倍体厚,颜色较二倍体稍深,表面较二倍体略显粗糙,通过生根后对根的观察发现四倍体的根较二倍体的粗壮,根条数比二倍体的要少。
The mature seed of flax ShuangYa-7 as material, induction of flax polyploidy was conducted through flax seeds immersion of colchicines followed by sterile culture. Namely, the disinfected seeds were applied to induce the polyploidy after germination in perti dish, the polyploidy were induced by the treatment of colchicines. The results showed that the aberration rate of flax seeds is 40.7% while the seeds after germination in water for 36 h were treated with 0. 075% concentration of colchicines for 24 h. Through observation of the external morphology, the stem of tetraploid is stronger than that of diploid, the leaf of tetraploid is thicker than that of diploid and the color of tetraploid is darker than that of diploid, the surface of tetraploid is rougher than that of diploid, the root of tetraploid is stronger than that of diploid and root number of branches are less than that of diploid.
出处
《激光生物学报》
CAS
CSCD
2013年第1期91-96,共6页
Acta Laser Biology Sinica
基金
黑龙江省农垦总局科技计划重点攻关项目(HNK11A-01-07-08)
黑龙江省自然科学基金(c201211)
黑龙江省研究生创新科研项目(YJSCX2012-257HLJ)
关键词
亚麻
秋水仙碱
诱导
多倍体
Flax
colchicines
induction
polyploid
