摘要
喜树碱 (CAM)诱导人类急性早幼粒细胞白血病细胞株 (HL- 6 0 )细胞 ,以高渗磷酸 -柠檬酸盐缓冲液(PC)处理 ,并同时用线性放大和对数放大进行 DNA含量分析。结果显示 ,在 DNA直方图上 ,可将亚 G1 凋亡峰和坏死细胞碎片峰有效地分开 ,并且不影响 G1 期细胞与 G2 期细胞 DNA含量间的线性关系。提示流式细胞术
HL 60 cells induced by camptothecin were treated with hypertonic phosphate citric acid buffer, and the DNA content was detected by using linear and logarithmic amplifier simultaneously. The results showed that the sub G 1 peak of apoptotic cells and the peak of necrotic cells could be separated efficiently, but the linearity between the DNA content of G 1 cells and G 2 cells wasn't affected. It was suggested that flow cytometric DNA content analysis was a major method for cell biological and clinical analysis.
出处
《同济医科大学学报》
CSCD
2000年第5期408-410,共3页
Acta Universitatis Medicinae Tongji
基金
国家自然科学基金!资助项目 (No.396 70 36 5
39730 2 70
3972 5 0 2 7)
卫生部基金资助项目! (No.2 0 2 - 0 1- 0 6 )
