摘要
建立了柱前衍生化反相高效液相色谱测定大鼠血浆中的丙二醛(MDA)的方法。以甲基丙二醛(Me-MDA)为内标,2,4-二硝基苯肼(DNPH)进行衍生化反应。采用ZORBAX SB C_(18)柱(250mm×4.6mm,5μm),流动相为0.2%冰醋酸溶液-乙腈(V/V)=50:50,流速为1.0mL/min,检测波长为310nm。丙二醛在2.5—60μmol/L范围内线性关系良好,r=0.9991,平均加标回收率为97.7%,相对标准偏差RSD为4.47%(n=6)。方法准确可靠,专属性好,样品用量少(50μL的血浆),适于大鼠血浆中丙二醛的含量测定。
A method based on reversed-phase high performance liquid chromatography （HPLC） with pre-column derivatization is established to determine malondialdehyde（MDA） in rat plasma with methyl malondialdehyde （Me-MDA） as an internal standard and 2, 4-dinitrophenylhydrazine （DNPH） as the derivatization reagent. The separation was performed on a ZORBAX SB C18 column （250mm）〈 4.6mm,5μm） with 0. 2% acetic acid-acetonitrile（50 ： 50,V/V） as the mobile phase at a flow rate of 1. 0mL/min. The detection wavelength was set at 310nm. The linear range of malondialdehyde was 2. 5-60μmol/L and the correlation coefficient was 0. 9991. The average recovery was 97.7% ,the relative standard deviation was 4.47% （n= 6）. The plasma volume of 50μL was sufficient for the determination of MDA. The method is accurate and reliable with good specificity,and suitable for the determination of MDA in rat plasma.
出处
《光谱实验室》
CAS
2013年第2期790-794,共5页
Chinese Journal of Spectroscopy Laboratory
基金
国家科技攻关计划"西部开发"重大项目(2005BA901A10)
广西植物研究所基本科研业务费资助项目(桂植业09003)
