摘要
目的:明确黄芪、三七及其配伍对萎缩性胃炎大鼠胃黏膜热休克蛋白70(heat shock protein 70,HSP70)及其转录因子GAF表达的影响.方法:清洁级Wistar大鼠54只,随机分为空白组、模型组、替普瑞酮组、黄芪组、三七组、黄芪+三七组.运用幽门弹簧配合高盐热淀粉糊灌胃法制备萎缩性胃炎模型后,分别给予空白组、模型组(生理盐水2mL/d)、黄芪组[黄芪水煎剂3.5g/(kg·d)]、三七组[三七粉冲剂0.7g/(kg·d)]、黄芪+三七组{黄芪三七溶液灌胃[其中黄芪3.5g/(kg·d)、三七0.7g/(kg·d)]}、替普瑞酮组[200mg/(kg·d)]灌胃.连续给药1mo后,取大鼠胃组织,经量子点免疫荧光组织化学方法测定各组大鼠胃黏膜中HSP70蛋白及其转录因子GAF表达量.结果:同模型组相比,黄芪组、三七组、黄芪+三七组以及替普瑞酮组胃黏膜中HSP70的表达有显著性差异(P=0.0000,0.0269,0.0020,0.006);同时上述各组HSP70转录因子GAF的表达,亦显著性高于模型组(P=0.0000,0.0000,0.00100.0052);黄芪组HSP70表达高于三七组(P=0.0134),但HSP70转录因子GAF的表达在两组间无统计学差异.结论:黄芪、三七及其配伍可以明显改善萎缩性胃炎大鼠胃黏膜状态;通过诱导并增加转录因子GAF的表达,促进热休克蛋白70的表达,可能是黄芪、三七治疗萎缩性胃炎的作用途径之一.
AIM： To evaluate the effect of astragalus, Panax notoginseng and their mixture on expression of heat shock protein 70 （HSP70） and GAF in atro- phic gastritis in rats. METHODS： Fifty-four healthy male Wistar rats were randomly divided into six groups： control group, model group, teprenone group, astraga-lus group, Panax notoginseng group and astrag- alus plus Panax notoginseng group. Atrophic gastritis was induced by implanting a pylorus spring and intragastrically administering hot salty starch paste. The control and model groups were given normal saline （2 mL） daily, while other groups were infused with water decoction of astragalus containing crude drug 3.5 g/（kg. d）, the Panax notoginseng powder containing crude drug 0.7 g/（kg.d）, Panax notoginseng powder and astragalus water decoction, and teprenone water suspension containing teprenone 200 mg/ （kg. d） for one month by gavage, respectively. The expression of heat shock protein 70 and GAF in the rat gastric mucosa was measured using quantum dot immunofluorescence histo- chemical technology.RESULTS： HSP70 protein expression in the astragalus, Panax notoginseng, astragalus plus Panax notoginseng and teprenone groups was significantly increased （all P 〈 0.05） compared to the model group. The expression of GAF in the astragalus, Panax notoginseng, astragalus plus Panax notoginseng and teprenone groups was also increased significantly compared to the model group （all P 〈 0.01）. Although the ex- pression of HSP70 in the astragalus group was higher than that in the Panax notoginseng group （P 〈 0.05）, there was no significant difference in the expression of GAF between the two groups （P 〉 0.05）.CONCLUSION： Astragalus, Panax notoginseng and their combination can improve mucosal atrophy in rats with atrophic gastritis by increas- ing GAF and HSP70 expression. GAF and HSP70 may be potential therapeutic targets for atrophic gastritis.
出处
《世界华人消化杂志》
CAS
北大核心
2013年第7期559-566,共8页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.30973741
北京中医药大学创新团队基金资助项目
No.2011-CXTD-24~~
