摘要
目的探讨乙型肝炎ｅ抗原（ＨＢｅＡｇ）作为围术期乙型肝炎病毒（ＨＢＶ）传播监控指标的可行性。方法使用套式ＰＣＲ（ｎＰＣＲ）极量稀释方法，以ＨＢＶ感染剂量（ＩＤ）为参比单位，ＨＢｅＡｇ与稀释血量为分类标准，评价ＨＢｅＡｇ对ＨＢｓＡｇ阳性血液传染性的影响。结果１５６例ＨＢｓＡｇ阳性者中，３１例（１９ ．９％）ＨＢｅＡｇ阳性，１１５例（７３．７％）ＨＢＶＤＮＡ阳性， ＨＢＶ传染性为 ０～１０９ ＩＤ／ｍｌ。与ＨＢｓＡｇ相比，ＨＢｅＡｇ对围术期 ＨＢＶ传播的预示作用更好。与 ＨＢｅＡｇ阴性血液相比，ＨＢｅＡｇ阳性血液的ＨＢＶ携带率更高，ＨＢＶ的传染性更强，传播所需的血量更少，对特异性预防的效果更差。结论在外科医生普遍进行ＨＢＶ特异性免疫的前提下，ＨＢｅＡｇ较ＨＢｓＡｇ更适合作为围术期ＨＢＶ传播的监控指标。
OBJECTIVE TO investigate whether it is feasible that HBeAg in place of HBsAg serves as a supervising and control- ling index of HBV spread during operation. METHODS Nested PCR limiting dilution analysis serves as a method. HBV infec- tive dose serves as a unit. HBeAg and bloody dose serve as a grading standard. All of the above means used to evaluate the in- fluence that HBeAg does to the infectivity of HBsAg positive blood. RESULTS One hundred and fifty six operated patients of HBsAg positive have been investigated. Thirty one of them (19. 9% ) are HBeAg positive.One hundred and fifteen(73. 7% ) are HBV DNA positive. HBV infectivity unit is 0-109 infective dose/ml. HBeAg is a kind of better index of HBV spread during operation than HBsAg. The number of HBeAg positive patients that need intensive prophylaxis is less than that of HBsAg positive patients. It is more accurate than HBsAg that HBeAg is used to indicate prophylaxis. HBV positive ratio of HBeAg positive blood is higher than HBeAg negative one. The infectivity of HBeAg positive blood is much stronger than HBeAg negative one. Quantity of HBeAg positive blood that HBV spread need is less than that of HBeAg negative one. The efficacy of special prophy- laxis to HBeAg positive blood is much are than that of HBeAg negative one. CONCLUSIONS By using hepatitis B vaccine in susceptible surgeons, HBeAg serving as a supervising and controlling index of HBV spread during operation is more suitable than HBsAg.
出处
《中华医院感染学杂志》
CAS
CSCD
2000年第4期244-246,共3页
Chinese Journal of Nosocomiology
