摘要
目的:建立超高效液相色谱(UPLC)同时测定脑心通胶囊中羟基红花黄色素A、芍药苷、阿魏酸、丹酚酸B、藁本内酯5个主要化学成分的方法。方法:采用WATERS ACQUITY UPLC BEH C18色谱柱(2.1 mm×100 mm,1.7μm),流动相为0.5%甲酸-乙腈梯度洗脱,流速为0.3 mL·min-1,检测波长400 nm(羟基红花黄色素A)、235 nm(芍药苷),280 nm(丹酚酸B),324nm(阿魏酸和藁本内酯)。结果:本方法可在24 min内完成一次色谱分析,各主要成分色谱峰之间有良好的分离度,羟基红花黄色素A、芍药苷、阿魏酸、丹酚酸B、藁本内酯5个成分质量浓度分别在0.970～9.70 mg·L-1(r=0.9993)、3.38～33.8 mg·L-1(r=0.9995)、0.336～3.36 mg·L-1(r=0.9996)、2.15～21.5 mg·L-1(r=0.9999)、1.46～14.6 mg·L-1(r=0.9998)的范围内与峰面积呈良好的线性关系,方法的平均回收率(n=6)分别为99.47%、101.3%、98.45%、98.54%、99.02%,精密度、重复性的RSD均小于2.0%。结论:本方法能同时测定脑心通胶囊中5个主要化学成分,可较全面地检测脑心通胶囊的质量。
Objective： To establish a UPLC method for simultaneous determination of five compounds（ hydroxysaf- flor yellow A, paeoniflorin, ferulic acid, salvianolic acid B, ligustilide） in Naoxintong capsules. Methods： The five compounds in Naoxintong capsules were simultaneously determined by UPLC with WATERS ACQUITY UPLC BEH C18 column（2. 1 mm × 100 mm,1.7 μm）. The mobile phase consisted of 0.5% formic acid aqueous -acetonitrile with a gradient elution program and the flow rate of 0. 3 mL ~ rain - ~, the determination wavelengths were 400 nm for hydroxysafflor yellow A,235 nm for paeoniflorin,280 nm for salvianolie acid B ,and 324 nm for ferulic acid and li- gustilide. Results： The five components were completely separated in 24 min at one time. There were good separa- tion degrees between each chromatic peak. The linearity was achieved in the range of 0. 970 - 9.70 mg · L- 1 （ r = 0. 9993） ,3.38 -33.8 mg · L-1（r =0. 9995） ,0. 336 -3.36 mg · L-1 （r =0. 9996） ,2. 15 -21.5 mg · L-1（r- 0. 9999 ） , 1.46 - 14.6 mg ·L - 1 （ r = 0. 9998 ） for hydroxysafflor yellow A, paeoniflorin, ferulic acid, salvianolic acid B, ligustilide, respectively. The average recoveries （ n = 6 ） were 99.47 %, 101.3 %, 98.45 %, 98.54% , 99.02 % , re- spectively. The RSDs of precision and reproducibility were all less than 2.0%. Conclusion： The method can simul-taneously determine the five major chemical compounds in Naoxintong capsules, and can be used for overall quality control of Naoxintong capsules.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2013年第3期414-418,共5页
Chinese Journal of Pharmaceutical Analysis
基金
国家重大新药创制专项:中药大品种脑心通胶囊技术改造研究,课题编号:2011ZX09201-201-26(IMB2009011)
