摘要
目的观察亚抑菌浓度（亚-MIC）苦参碱及与红霉素联用后对表皮葡萄球菌生物被膜的抑制作用以及对表皮葡萄球菌生物被膜形态学变化的影响。方法连续稀释法测定苦参碱和红霉素对表皮葡萄球菌的MIC；棋盘格法评价苦参碱和红霉素联用后对表皮葡萄球菌悬浮菌的抗菌活性；体外构建表皮葡萄球菌生物被膜，XTr减低法评价亚-MIC苦参碱及其与红霉素联用对表皮葡萄球菌生物被膜内细菌代谢及初始黏附能力的影响，扫描电镜观察用药后表皮葡萄球菌形态和生物被膜结构改变。结果红霉素对表皮葡萄球菌的MIC为7．8125μg／ml，苦参碱对表皮葡萄球菌的MIC大于1000μg／ml；两药联用时对表皮葡萄球菌悬浮菌有协同作用（FIC指数〈O．5）。亚一MIC苦参碱对表皮葡萄球菌黏附作用无显著影响，与红霉素合用效果优于二者单独使用；亚．MIC苦参碱对表皮葡萄球菌生物被膜菌的代谢和形态均有抑制作用，其与红霉素联用时的作用弱于二者单独使用。结论亚-MIC苦参碱与红霉素对表皮葡萄球菌生物被膜的形成有显著影响；二者联合使用对表皮葡萄球菌悬浮菌及被膜菌黏附的抑制有协同作用，对表皮葡萄球菌生物被膜菌的代谢活性及形态影响均无协同作用。
Objective To observe the inhibitory effects of sub-MIC matrine alone and in combination with erythromycin on Staphylococcus epidermidis biofilms and their influences on morphological changes of the biofilms. Methods Minimum inhibitory concentrations （MIC） of matrine and erythromycin against Staphylo- coccus epidermidis were determined by the serial dilution method, antibacterial activity of matrine combined with erythromycin against planktonic S. epidermidis was evaluated by the checkerboard method. S. epidermidis bio- films were constructed in vitro, XTT reduction assay was used to evaluate influences of sub-MIC matrine alone and in combination with erythromycin on metabolism and adhesion of S. epidermidis biofilms, and scanning elec- tronic microscope （SEM） was applied to observe the morphological and the structural changes of the biofilms. Results The MIC of erythromycin to S. epidermidis was 7. 8125 Ixg/ml, while the MIC of matrine was greater than 1000 p^g,/ml, besides, a synergistic effect between erythronmycin and matrine on planktonic S. epidermidis was shown （ FIC〈0.5 ）. The sub-MIC matrine had no significant inhibitory effect on adhesion of S. epidermidis, and also the combination of the two agents was better than was used alone. However, the sub-MIC matrine had inhibitory effects on metabolism and morphology of S. epidermidis biofilms, and the combination of the two agents was weaker than was used alone. Conclusion Both the sub-MIC matrine and erythromyein had a significant in- hibitory effect on S. epidermidis biofilm formation. Combination of the two agents showed synergistic effects on plankton and adhesion of S. epidermidis, but showed no synergistic effect on metabolism and morphology of the biofilms.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2013年第2期86-90,共5页
Chinese Journal of Microbiology and Immunology
基金
安徽省教育厅自然科学研究重点项目（KJ2010A214,KJ20132163）
关键词
苦参碱
表皮葡萄球菌
生物被膜
亚抑菌浓度
药物协同
Matrine
Staphylococcus epidermidis
Biofilm
Sub-minimum inhibitory concentration（MIC）
Synergistic effect
