摘要
将192只清洁级SD大鼠随机分为6组,各组分别饮用添加硼0、40、80、160、320、640mg/L蒸馏水60d,2次取材,分别测定血清抗氧化功能及血清胆固醇、葡萄糖、总蛋白、ALP、ALT、AST、钙、镁、磷等9项血清生化指标。结果显示:与对照组相比,(1)65d试验Ⅰ、Ⅲ、Ⅴ组雄鼠和Ⅰ、Ⅴ组雌鼠平均体质量(MB)显著或极显著降低(P<0.05或P<0.01);65d和95d试验Ⅴ组雄鼠和雌鼠平均日增重(ADG)和Lee’s指数均显著或极显著降低(P<0.05或P<0.01);95d试验Ⅴ组雄鼠和雌鼠MB和体长极显著降低(P<0.01);(2)65d试验Ⅲ组血糖、ALT和镁,Ⅳ组血钙及Ⅴ组血糖、ALP和血钙水平均显著或极显著降低(P<0.05或P<0.01);95d试验Ⅲ组血清胆固醇和血钙,Ⅳ组血清胆固醇、血磷和血钙及Ⅴ组血糖、ALT、AST、钙、磷和镁水平均显著或极显著降低(P<0.05或P<0.01);65d试验Ⅱ血钙,Ⅴ组血清胆固醇和AST水平,及95d试验Ⅴ组血清胆固醇则显著或极显著升高(P<0.05或P<0.01);(3)65d试验Ⅳ、Ⅴ组MDA含量,95d试验Ⅴ组MDA含量和GSH-Px活性,及95d试验Ⅱ～Ⅳ组SOD显著或极显著升高(P<0.05或P<0.01);65d试验Ⅴ组SOD、CAT、GSH-PX,95d试验Ⅲ组MDA含量,及Ⅳ组GSH-Px活性显著或极显著降低(P<0.05或P<0.01)。试验结果表明,饮水添加40～320mg/L硼对95、65d大鼠血清抗氧化能力和血清胆固醇、葡萄糖及钙、磷、镁离子水平产生不同的影响,尤其对血清抗氧化能力影响明显,而对血清蛋白质含量和血清酶活性没有显著影响;添加640mg/L硼对大鼠血清各项生化指标及抗氧化能力均有明显不良影响甚至毒性作用,机体胆固醇、脂类、糖类及离子代谢发生紊乱,抗氧化能力明显下降。
In order to the effects of different levels of boron supplemented in drinking water on the biochemical index and antioxidant capacity of serum in rats. One hundred and ninety two clean level SD rats were randomly divided into six groups：control group and experiment group Ⅰ , Ⅱ , Ⅲ, IV and V Treated rats drank the water supplemented with 40,80,160,320 and 640 mg/L bo- ron. At the 30 d and 60 d of experiment period, 16 rats （n=8,male=female） from each group were selected and the serum samples were obtained. Then serum biochemical indexes （cholester- ol, glucose, total protein, ALP, ALT, AST, Caz＋ , Mg^2＋ and P） and the antioxidant capacity were detected. The results were as follows ： （1） compared with the control group, the mean weight of the female rats in group Ⅰ and Ⅴ ,and the male rats in group Ⅰ , Ⅲ and V were significantly de- creased at 65 d （P〈0.05 or P〈0.01） ;the average daily gain （ADG） and Lee＇s index of male and female rats in group Vwere significantly decreased at 65 d and 95 d（P〈0.05 or P〈0.01）;the MB and stem length of male and female rats in experiment group V were significantly decreased at 95 d （P〈0.01）. （2） In the 65 day-old rats,the level of serum cholesterol,ALT and magnesium ingroup Ⅲ ,the blood calcium in group Ⅳ ,the glucose,ALP and calcium in groupV were all signifi- cantly decreased （P〈0.05 or P〈0.01）;in the 95 day-old rats,the level of serum cholesterol and calcium in group Ⅲ, serum cholesterol, calcium and phosphorus in group Ⅳ,the serum glucose, ALT,AST,calcium,phosphorus and magnesium in group V were also significantly decreased （P 〈0.05 or P〈0.01）;but the level of serum calcium in group Ⅱ , serum cholesterol and AST in groupV at 65 d,and the level of serum cholesterol in groupVwere significantly increased at 95 d （P〈0.05 or P〈0.01）. （2） In the 65 day-old rats,the concentration of MDA in group Ⅳ and Ⅴ were significantly increased （P〈0.05 or P〈0.01）, and the activity of SOD, CAT, GSH-Px in group V were significantly decreased （P〈0.05 or P〈0.01） ;in the 65 day-old rats,the concentra- tion of MDA and the activity of GSH-Px in groupV ,the activity of SOD in group Ⅱ-Ⅳ were sig- nificantly increased,and the concentration of MDA in group Ⅲ , the activity of GSH-Px in group Igwere significantly decreased （P〈0.05 or P〈0.01）. The results indicated that the boron sup- plemented in distilled water with from 40 to 320 mg/L could have obvious and different effects on the serum antioxidant capacity and the concentration of cholesterol, glucose, calcium, phosphorus and magnesium of serum in 65 and 95-day-old rats,and have some influences on the serum protein levels and the serum enzyme activity of rats,in which the effects on serum antioxidant capacity was especially obvious. But the boron showed obvious harmful and even toxic effects on the bio- chemical indexes and the antioxidant capacity of serum in rats supplemented with 640 mg/L of bo- ron. In this circumstance, the body metabolism of cholesterol, lipid, sugar and serum ions displayed disorder,and the antioxidant capacity decreased obviously.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第3期486-492,共7页
Chinese Journal of Veterinary Science
基金
教育部科学技术研究重点资助项目(210095)
安徽省自然科学基金资助项目(090411001)
安徽高校省级自然科学研究重点资助项目(KJ2009A026Z)
安徽科技学院重点学科基金资助项目(AKXK20101-2)
关键词
硼
大鼠
血清
生化指标
抗氧化能力
boron
rat
serum
biochemical index
antioxidant ability
