摘要
目的:建立液-液萃取、GC/μECD气相色谱测定生物检材中佐匹克隆的方法。方法:以家兔为模型动物,500 mg佐匹克隆标准品灌胃家兔,6.5小时后处死,取其胃、肝、血、脑、心脏、尿,采用乙醚液-液萃取提取,Rtx-5毛细管柱分离,电子捕获检测器检测,标准曲线法定量。结果:该方法灵敏度达0.02μg/mL,在0.05-50μg/mL范围内线性关系良好;低、中、高3种浓度的加样回收率分别为91.7%、99.5%、94.4%,相对标准偏差(RSD)均小于5%;佐匹克隆在家兔脏器中的浓度分别为7.31μg/mL(血液)、7.63μg/g(肝脏)、7.96μg/g(脑)、14.67μg/g(心脏)、32.18μg/g(胃组织)、42.65μg/mL(尿液),在各脏器中呈特异性分布。结论:该法操作简单、灵敏度高,结果可靠,完全满足临床检测及公安机关在办理佐匹克隆麻醉案件中的需要。
Objective： To develop a method for determination of zopiclone in biological matrices by liquid-liquid extraction and GC/μECD. Methods： The model animals-rabbits were sacrificed 6.5 hours after gastric perfusion of 500mg zopiclone. The biological matrices including stomach, liver, blood, brain, heart and urine were extracted by aether, separated by Rtx-5 columm and then detected by μECD detector. Results： The detection limit of this method was 0.02μg/mL.It had a good linear relationship with zopiclone in the range of 0.05-50 μg/mL.The mean recoveries of low, middle and high concentrations were 91.7 %, 99.5 %, 94.4 %, and all of the relative standard deviation （RSD） were less than 5 %.The concentrations of zopiclone in the tissues were 7.31 μg/mL （blood）, 7.63μg/g（liver）, 7.96 μg/g（brain）, 14.67 μg/g（heart）, 32.18 μg/g（stomaeh）and 42.65μg/mL（urine） respectively. This showed zopiclone was differentially distributed in the tissues. Conclusions： The method is easy to operate, sensitive and reliable, and could be applied to clinical determination and forensic.identification.
出处
《现代生物医学进展》
CAS
2013年第2期225-227,262,共4页
Progress in Modern Biomedicine
基金
公安部应用创新计划项目[2005YYCXHNST092]
