摘要
目的:研究15株多重耐药金黄色葡萄球菌耐药基因和肠毒素基因定位特征。方法:对15株多重耐药金黄色葡萄球菌质粒消除前后,进行药敏试验和肠毒素测定比较,从而对耐药基因和肠毒素基因进行定位。结果:15株多重耐药金黄色葡萄球菌耐药基因大多数位于质粒上,由质粒介导的耐药基因占70.6%,由染色体编码的耐药基因占29.4%。而对于肠毒素基因,发现SEA、SEC位于染色体上,SED位于质粒上,而SEB有位于染色体或质粒上。结论:耐药基因、肠毒素基因可以定位到染色体或质粒上,且质粒在决定耐药性中起主要作用。
Objective ：To investigate the resistant gene and enterotoxin gene of 15 strains of muhiple - resistant staphylococcus aureus from food. Methods： The resistant genes and enterotoxin genes of staphylococcu~ aurcus were detected before and after elimination of the plasmids and finding the location of those genes. Results： The resistant gene of 15 strains of staphylococcus aureus were mainly located in plasmids, the 70.6% of resistant gene was media- ted by the plasmid and 29.4% of the resistant gene was mediated by the chromosome;the genetic locus of SEA, SEC were located in chromosome,and that of SED was located in plasmids, SEB was located in chromosome or plasmids. Conclusion： Antibiotics resistant gene and enterotoxin gene are located in both plasmids and chromosome. And plasmids play an important role in determining the resistant gene and enterotoxin gene.
出处
《中国卫生检验杂志》
北大核心
2013年第2期498-499,502,共3页
Chinese Journal of Health Laboratory Technology
