摘要
目的:探索快速查明食物中毒病原菌的途径,为事件处置提供及时、准确的实验室依据。方法:采用实时荧光PCR和国标方法分别对一起食物中毒所采集的生物样品进行常见食源致病菌检测。结果:11件样品经实时荧光PCR法检出沙门菌特异性DNA核酸阳性7份,阳性率为63.6%;11件样品经国标法细菌学分离培养检出5株沙门菌,阳性率为45.5%。所有样品经两种方法均未检出副溶血性弧菌、志贺菌、霍乱弧菌等其它致病菌。结论:通过实时荧光PCR与国标法细菌学检测,均证实此次食物中毒的病原菌为沙门菌。实时荧光PCR与国标法的联合应用对致病菌检测是一种优势互补,适合在突发公共卫生事件的病原菌检测中推广应用。
Objective：To provide timely and accurate laboratory basis in event handling, based on the develop- ment of rapid identification for food poisoning pathogen. Methods： The foodborne bacterial pathogens of the collect- ed biological samples in food poisoning were inspected using real - time fluorescence PCR and national standard method. Results： Through real - time fluorescence PCR, detection results of 11 samples showed that salnumella specific DNA nucleic acid was positive in 7 cases and positive rate was 63.6%. In addition, detection results of 11 samples elucidated that salmonella was positive in 5 cases （45.5%） using bacteriological culture in national stand- ard method. Moreover, vibrio parahaemolyticus, shigeUa, vibrio cholerae and other pathogens were not found in all samples by both two methods. Conclusion： Real -time fluorescence PCR and national standard method show that pathogenic bacteria of food poisoning are salmonella. Furthermore, real - time fluorescence PCR and national stand- ard method are complementary. Therefore, they can be widely used to detect pathogenic bacteria in emergent events of public health.
出处
《中国卫生检验杂志》
北大核心
2013年第2期372-374,共3页
Chinese Journal of Health Laboratory Technology
关键词
实时荧光PCR
国标方法
检测
食物中毒
致病菌
沙门菌
Real - time fluorescence PCR
National standard method
Inspect
Food poisoning
Foodbornebacterial pathogens
Salmonella
