摘要
目的建立多重PCR方法同时检测水产品中沙门菌、副溶血性弧菌和单核细胞增生性李斯特菌。方法分别以沙门菌invA基因、副溶血性弧菌toxR基因和单核细胞增生性李斯特菌iap基因为靶基因,建立并优化了同时检测水产品中这3种致病菌的多重PCR体系,扩增产物分别为495bp(invA)、368bp(toxR)和660bp(iap)。结果建立的多重PCR方法可以简便、快速、灵敏地实现水产品中沙门菌、副溶血性弧菌和单核细胞增生性李斯特菌的同时检测,人工污染水产品检测限为10cfu/ml。结论为无公害水产品食源性致病菌的快速检测提供了理想手段,有良好的应用前景。
Objective To develop a rapid multiplex PCR（m-PCR） assay for simultaneously detection of three foodborne pathogens in aquatic products.Methods The invasion protein gene（invA） of Salmonella spp.,toxR gene（toxR） of Vibrio parahaemolyticus and invasion-associated protein p60 gene（iap） of Listeria monocytogenes were used as the gene targets.Results The multiplex PCR assay could be specific and rapid,and the detection limits were 10 cfu/ml when the artificially contaminated aquatic products were incubated at 37℃ for 10h.Conclusion The multiplex PCR assay developed in this study could provide a cost-effective supplement of conventional microbiological methods for routine monitoring of food.
出处
《卫生研究》
CAS
CSCD
北大核心
2008年第5期602-605,共4页
Journal of Hygiene Research
基金
广东省重大科技攻关项目(No.2003A20507)
2006年粤港关键领域重点突破项目(No.2006A25005001)
